Human LILRA1/CD85i/LIR-6 Antibody

Catalog #: MAB30852 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB30852-100
MAB30852-SP

LILRA1/CD85i/LIR-6 in THP-1 Human Cell Line and PBMCs.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRA1/CD85i/LIR-6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRA1/CD85i/LIR-6 in direct ELISAs. In direct ELISAs, no cross-reactivity with human LILRA2, LILRA3, LILRA4, LILRA5, LILRA6, LILRB2 and LILRB3 was observed.

Source

Monoclonal Mouse IgG₁ Clone # 586439

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived human LILRA1/CD85i/LIR-6
Pro17-Asn461
Accession # O75019

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

View Larger

LILRA1/CD85i/LIR-6 in THP-1 Human Cell Line and PBMCs. LILRA1/CD85i/LIR-6 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line (left panel, positive stain) and human peripheral blood mononuclear cells (PBMCs; right panel, negative stain) using Mouse Anti-Human LILRA1/CD85i/LIR-6 Monoclonal Antibody (Catalog # MAB30852) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA1/CD85i/LIR-6

Leukocyte Immunoglobulin-like Receptor 6 (LIR-6), also called LILRA1 or CD85i, is a glycoprotein member of the LIR family of leukocyte Ig-like receptors. As an activating LIR, LIR-6 has a short cytoplasmic tail and a charged aa within the TM domain which interacts with FcR gamma. Both four (LIR-6a) and two (LIR-6b) Ig-like domain forms are expressed by monocytes and B cells. Human LIR-6 has no non-primate ortholog, but shows up to 80% aa identity with human ILT-1c and KIR-p91/PIR-B. A mouse homolog, gp49B2, has structural similarity but limited sequence identity.

Long Name

Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 1

Entrez Gene IDs

11024 (Human)

Alternate Names

CD85 antigen-like family member I; CD85i antigen; CD85i; leukocyte immunoglobulin-like receptor subfamily A member 1 soluble isoform; leukocyte immunoglobulin-like receptor subfamily A member 1; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1; LILRA1; LIR6; LIR-6; LIR-6Leukocyte immunoglobulin-like receptor 6; LIR6MGC126563