Human LILRA3/CD85e Alexa Fluor® 488-conjugated Antibody Summary
Arg18-Glu439
Accession # Q8N6C8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of LILRA3/CD85e in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Goat Anti-Human LILRA3/CD85e Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2574G) or (B) Normal Goat IgG Alexa Fluor 488 Control (Catalog # IC108G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
Background: LILRA3/CD85e
Human LIR-4 (Leukocyte Ig-like receptor #4; also ILT6 and LILRA3) is an Ig superfamily member that belongs to the leukocyte receptor complex/cluster. Mature LIR-4 is 416 amino acids in length and contains four C2-type Ig-like domains. Unlike other LIR family members, LIR-4 is actively secreted. At least one alternate splice form is known which shows a 64 amino acid deletion in the C-terminal half of the second Ig-like domain. Multiple alleles exist in human, and 5-10% of the population have a defective gene which produces no LIR-4 product. Rodent LIR-4 has not been reported.
Product Datasheets
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Citation for Human LILRA3/CD85e Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Upregulated expression of leukocyte immunoglobulin‑like receptor A3 in patients with severe aplastic anemia
Authors: Hong Yu, Hui Liu, Yang Zhao, Huaquan Wang, Chunyan Liu, Weiwei Qi et al.
Experimental and Therapeutic Medicine
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