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Human LIMD1 Antibody

Catalog #: MAB8494
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8494
MAB8494-SP
Detection of Human LIMD1 by Western Blot.
3 Images
Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LIMD1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LIMD1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 919330
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human LIMD1
Met1-Val143
Accession # Q9UGP4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human LIMD1 antibody by Western Blot. View Larger

Detection of Human LIMD1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LIMD1 Monoclonal Antibody (Catalog # MAB8494) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LIMD1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry LIMD1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

LIMD1 in HeLa Human Cell Line. LIMD1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human LIMD1 Monoclonal Antibody (Catalog # MAB8494) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry LIMD1 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

LIMD1 in Human Breast Cancer Tissue. LIMD1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human LIMD1 Monoclonal Antibody (Catalog # MAB8494) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIMD1

Human LIM Domain-containing Protein 1 (LIMD1) is a ubiquitous tumor suppressor 72 kDa protein which has three LIM zinc-binding domains. Via its LIM domains LIMD1 interacts with TRAF6, SNAI2/SLUG  and SCRT1 (via SNAG domain) proteins. It also interacts with SQSTM1 and RB1, EIF4E, AGO1, AGO2, DCP2, DDX6, LATS1, LATS2, EGLN1/PHD2, EGLN2/PHD1 and EGLN3/PHD3 proteins. LIMD1 interacts with isoform 1 and isoform 3 of VHL and with SNAI1 proteins. LIMD1 localized predominantly in the cytoplasm but shuttles between cytoplasm and nucleus where it can be often detected: strong nuclear expression is indicative for a low-tumor grade and a better patient prognosis. Down-regulation  of LIMD1 is in the nuclei of neoplastic cells, is the indication for a poor prognosis of breast cancer. The correlation exists between LIMD1 and IRF4 in cell lines derived from lymphomas. In ulcerative epithelium it was found that LIMD1 has a reduced nuclear expression. LIMD1 is encoded at chromosome 3p21.3, a region which is deleted in many solid tumors and LIMD1 is down-regulated in the majority of human lung cancer samples. LIMD1 also is a regulator of both bone osetoclast and bone osteoblast development and function. Human LIMD1 shares 77% aa sequence identity with both rat and mouse LIMD1.         

Long Name
LIM Domains Containing 1
Entrez Gene IDs
8994 (Human); 29806 (Mouse); 316101 (Rat)
Alternate Names
LIM domain-containing protein 1; LIM domains containing 1; LIMD1

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Citations for Human LIMD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A single-cell regulatory map of postnatal lung alveologenesis in humans and mice
    Authors: Thu Elizabeth Duong, Yan Wu, Brandon Chin Sos, Weixiu Dong, Siddharth Limaye, Lauraine H. Rivier et al.
    Cell Genomics
  2. SKI activates the Hippo pathway via LIMD1 to inhibit cardiac fibroblast activation
    Authors: Natalie M. Landry, Sunil G. Rattan, Krista L. Filomeno, Thomas W. Meier, Simon C. Meier, Sarah J. Foran et al.
    Basic Research in Cardiology

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