Human LINGO-2 APC-conjugated Antibody

Catalog #: FAB36791A Datasheet / COA / SDS

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FAB36791A-025

Detection of LINGO‑2 in A549 Human Cell Line by Flow Cytometry.

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Human LINGO-2 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human LINGO-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LINGO-1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 382007

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LINGO-2
Cys28-Leu542
Accession # Q7L985

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LINGO‑2 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human LINGO-2 APC-conjugated Monoclonal Antibody (Catalog # FAB36791A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of LINGO‑2 in HEK293 Human Cell Line Transfected with Human LINGO-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human LINGO-2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LINGO-2 APC-conjugated Monoclonal Antibody (Catalog # FAB36791A). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LINGO-2

Human LINGO-2 (LRR and Ig domain-containing, Nogo Receptor-interacting protein 2), also known as Leucine-Rich Repeat Neuronal 6C (LRRN6C) or LERN3), type I transmembrane protein in the neuronal leucine-rich repeat family. These proteins have a signal peptide, 12 extracellular leucine-rich repeats flanked by N-terminal and C-terminal cysteine-rich sequences, an immunoglobulin-like domain, a transmembrane domain and a short cytoplasmic tail. An alternate start site may exist at Met148 of the precursor. Human LINGO-2 is a highly conserved, 606 amino acid (aa) protein that shares 99% and 98% aa sequence identity with canine and mouse LINGO-2, respectively. LINGO-2 presumably functions outside the CNS with little involvement by p75/NgR1.

Long Name

Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 2

Entrez Gene IDs

158038 (Human); 242384 (Mouse); 313156 (Rat)

Alternate Names

LERN3; LERN3FLJ31810; leucine rich repeat and Ig domain containing 2; leucine-rich repeat and immunoglobulin-like domain-containing nogoreceptor-interacting protein 2; Leucine-rich repeat neuronal protein 3; Leucine-rich repeat neuronal protein 6C; LINGO2; LINGO-2; LRRN6C; LRRN6Cleucine rich repeat neuronal 6C