Human Lipin 1 Antibody Summary
Asp448-Leu574
Accession # Q14693
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Lipin 1 in Human Adipocytes. Lipin 1 was detected in immersion fixed human mesenchymal stem cell-derived adipocytes using Goat Anti-Human Lipin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3885) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipin 1
Lipin 1 is a 100 kDa phosphatidate phosphatase. Mammalian Lipin 1 has been identified as an enzyme that regulates fat metabolism. There is one human Lipin-1. In mice, two Lipin-1 isoforms have been reported, alpha and beta. Lipin 1 alpha is a nuclear protein and is 96% and 98 % identical to human Lipin 1 and rat Lipin 1 alpha, respectively. Lipin 1 beta is cytoplasmic.
Product Datasheets
Citations for Human Lipin 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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UVB-dependent inhibition of lipin-1 protects against proinflammatory responses in human keratinocytes
Authors: M Chae, ED Son, IH Bae, EG Cho, HJ Kim, JY Jung
Exp. Mol. Med., 2020-02-21;0(0):.
Species: Human
Sample Types: Cell Culture Lysates
Applications: Western Blot -
HIF-1-dependent lipin1 induction prevents excessive lipid accumulation in choline-deficient diet-induced fatty liver
Authors: T Arai, M Tanaka, N Goda
Sci Rep, 2018-09-21;8(1):14230.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ChIP, Western Blot -
Degradation of human Lipin-1 by BTRC E3 ubiquitin ligase
Authors: K Ishimoto, A Hayase, F Kumagai, M Kawai, H Okuno, N Hino, Y Okada, T Kawamura, T Tanaka, T Hamakubo, J Sakai, T Kodama, K Tachibana, T Doi
Biochem. Biophys. Res. Commun., 2017-05-05;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Dietary cholesterol reduces plasma triacylglycerol in apolipoprotein e-null mice: suppression of lipin-1 and -2 in the glycerol-3-phosphate pathway.
Authors: Obama T, Nagaoka S, Akagi K, Kato R, Horiuchi N, Horai Y, Aiuchi T, Arata S, Yamaguchi T, Watanabe M, Itabe H
PLoS ONE, 2011-08-09;6(8):e22917.
Species: Human, Mouse
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot -
Sterol-mediated regulation of human lipin 1 gene expression in hepatoblastoma cells.
Authors: Ishimoto K, Nakamura H, Tachibana K, Yamasaki D, Ota A, Hirano K, Tanaka T, Hamakubo T, Sakai J, Kodama T, Doi T
J. Biol. Chem., 2009-06-24;284(33):22195-205.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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