Human Lipocalin-1 Biotinylated Antibody

Catalog #: BAF1708 Datasheet / COA / SDS

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BAF1708

All Lipocalin-1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Lipocalin-1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Lipocalin-1 in Western blots. In this format, approximately 5% cross‑reactivity with rhLipocalin-2 and rmLipocalin-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Lipocalin-1 (R&D Systems, Catalog # 1708-PI)
His19-Asp176
Accession # P31025

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Lipocalin‑1 (Catalog # 1708-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-1

Lipocalin-1, also known as tear prealbumin or von Ebner’s gland protein (VEGP), is encoded by the LCN1 gene (1 - 3). It is a member of the Lipocalin superfamily that binds many different classes of lipophylic chemicals (4). Lipocalin-1 contains three sequence motifs similar to the cystatins, a superfamily of cysteine protease inhibitors (5). In fact, it has been suggested that Lipocalin-1 is a physiological inhibitor of cysteine proteases and plays a role in the control of inflammatory processes in oral and ocular tissues (5). rhLipocalin-1 corresponds to the mature and secreted protein. It is a weak inhibitor of cysteine proteases such as cathepsin V, which is similar to rhCystatin S.

References

Redl, B. et al. (1992) J. Biol. Chem. 267:20282.
Blaker, M. et al. (1993) Biochim. Biophys. Acta 1172:131.
Lassagne, H. and A.M. Gachon (1993) Exp. Eye Res. 56:605.
Redl, B. et al. (2000) Biochim. Biophys. Acta 1482:241.
van’t Hof, W. et al. (1997) J. Biol. Chem. 272:1837.

Entrez Gene IDs

3933 (Human)

Alternate Names

LCN1; lipocalin 1 (tear prealbumin); lipocalin 1-like 2; Lipocalin1; Lipocalin-1; MGC71975; PMFA; Tear lipocalin; Tear Prealbumin; TLC; TPVon Ebner gland protein; VEG protein; VEGP; VEGPlipocalin 1 (protein migrating faster than albumin, tear prealbumin)