Human LITAF Antibody Summary
Met1-Ala111
Accession # Q99732
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human LITAF by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human LITAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4695) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LITAF at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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LITAF in A431 Human Cell Line. LITAF was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human LITAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4695) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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LITAF in Human Small Intestine. LITAF was detected in immersion fixed paraffin-embedded sections of human small intestine using Goat Anti-Human LITAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4695) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of lymphocytes in lamina propria in intestinal villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LITAF
LITAF (LPS-induced TNF- alpha factor), initially identified through its interaction with the TNF-alpha promoter, is a transcription factor that contributes to the regulation of several inflammatory cytokines in response to LPS or p53 stimulation. LITAF interacts directly with LPS-induced STAT6(B) in the cytoplasm, this complex then translocates into the nucleus, where it significantly up-regulates the transcription of other inflammatory mediators such as, GRO, IL-1 alpha, TNF-alpha, MCP-2 and IFN-gamma. Phosphorylation of LITAF by p38 alpha via the TLR pathway is also required for nuclear translocation. Mutations in LITAF have been associated with CMT1C
(Charcot‑Marie-Tooth neuropathy type 1C) an autosomal dominant demyelinating form of peripheral neuropathy.
Product Datasheets
Citation for Human LITAF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Sequential CRISPR-Based Screens Identify LITAF and CDIP1 as the Bacillus cereus Hemolysin BL Toxin Host Receptors
Authors: Jie Liu, Zehua Zuo, Inka Sastalla, Chengyu Liu, Ji Yong Yong Jang, Yusuke Sekine et al.
Cell Host & Microbe
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