Human LMO2 Antibody

Catalog #: MAB2726 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2726
MAB2726-SP

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Human LMO2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LMO2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 313606

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human LMO2
Met1-Ile158
Accession # P25791

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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LMO2 in Human Tonsil. LMO2 was detected in formalin fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human LMO2 Monoclonal Antibody (Catalog # MAB2726) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LMO2

LMO2, also known as Rhombotin-2 and T-cell translocation protein-2, is a transcriptional co‑factor that is required for hematopoietic and endothelial development. It contains two LIM domains that are characterized by a zinc binding, cysteine rich motif consisting of two tandemly repeated zinc fingers. LMO2 does not interact directly with DNA but is involved in the assembly of multiprotein transcription factor complexes. Human and mouse LMO2 share 99% amino acid sequence homology.

Long Name

LIM Domain Only 2

Entrez Gene IDs

4005 (Human)

Alternate Names

Cysteine-rich protein TTG-2; LIM domain only 2 (rhombotin-like 1); LIM domain only protein 2; LMO2; LMO-2; RBTN2; RBTN2rhombotin-2; RBTNL1; RBTNL1rhombotin-like 1; RHOM2; RHOM2T-cell translocation protein 2; T-cell translocation gene 2; TTG2; TTG2rhombotin 2