Human LOX-1/OLR1 Antibody Summary
Ser61-Gln273
Accession # P78380
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of LOX‑1/OLR1 in THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line were treated for 72 hours with 50 ng/mL PMA then stained with Mouse Anti-Human LOX-1/OLR1 Monoclonal Antibody (Catalog # MAB1798, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LOX-1/OLR1
Lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1), also known as oxidized low-density-lipoprotein receptor-1 (OLR-1), is a type II transmembrane receptor belonging to the C-type lectin family (1). It also belongs to the functionally defined scavenger receptor (SR) superfamily, whose members share the common ability to bind and internalize modified forms of Low Density Lipoproteins (LDL) (2-4). LOX-1 is the first member of the class E scavenger receptor subfamily (SR-E). It binds and supports the internalization of multiple structurally unrelated macromolecules including oxidized LDL, advanced glycation end products (AGE), activated platelets, bacteria, apoptotic or aged cells, and heat shock proteins (5-7). LOX-1 has also been implicated as an intestinal receptor involved in the transcytosis of pancreatic bile salt-dependent lipase (8). The human LOX-1 gene encodes a 273 amino acid (aa) residue protein with a short N-terminal intracellular domain, a transmembrane domain, an extracellular stalk/neck region followed by a C-type lectin-like domain (CTLD) (1, 6). The CTLD, which is required for ligand recognition, contains the six conserved cysteine residues present in all C-type lectins, but lacks the Ca2+-binding residues found in classical C-type lectins. LOX-1 can be detected on activated endothelial cells, vascular smooth muscle cells, macrophages, intestinal cells, and dendritic cells (6-8). The expression of LOX-1 is induced by proinflammatory or proatherogenic stimuli, as well as by oxidized LDL itself and hemodynamic or oxidative stress. Human LOX-1 exists on the cell surface as covalent homodimers, which can further associate into non-covalent-linked oligomers (9). Cell surface LOX-1 can also be cleaved by yet unidentified proteases to release the soluble LOX-1 extracellular domain (6). Binding and endocytosis of oxidized LDL by LOX-1 induces oxidative stress, activates NF kappa B, and upregulates the expression of monocyte chemoattractant protein-1 and matrix metalloproteases (5-9). LOX-1-dependent oxidized LDL uptake also induces apoptosis by inducing the expression of the pro-apoptotic Bax and downregulation of the anti-apoptotic Bcl-2 (10). Oxidized LDL plays a key role in the pathogenesis of atherosclerosis and endothelial dysfunction. Blockade of LOX-1 functions may turn out to be a suitable target for the therapeutic intervention of atherosclerosis.
- Sawamura, T. et al. (1997) Nature 386:73.
- Daugherty, A. et al. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs. 2:223.
- Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
- Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
- Jono, T. et al. (2002) FEBS Lett. 511:170.
- Kume, N. et al. (2001) Curr. Opin. Lipidol. 12:419.
- Delneste, Y. et al. (2002) Immunity 17:353.
- Bruneau, N. et al. (2003) Mol. Biol. Cell 14:2861.
- Xie, Q. et al. (2004) DNA and Cell Biol. 23:111.
- Chen, J. et al. (2003) Circ. Res. 94:370.
Product Datasheets
Citations for Human LOX-1/OLR1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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The AGP-PPAR gamma axis promotes oxidative stress and diabetic endothelial cell dysfunction
Authors: Ryoko Tsukahara, Hisao Haniu, Yoshikazu Matsuda, Tamotsu Tsukahara
Molecular and Cellular Endocrinology
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SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis
Authors: L Huang, KL Chambliss, X Gao, IS Yuhanna, E Behling-Ke, S Bergaya, M Ahmed, P Michaely, K Luby-Phelp, A Darehshour, L Xu, EA Fisher, WP Ge, C Mineo, PW Shaul
Nature, 2019-04-24;0(0):.
Species: Human
Sample Types: Whole Cells
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Increased Levels of Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 in Ischemic Stroke and Transient Ischemic Attack
Authors: T Skarpengla, M Skjelland, XY Kong, K Skagen, S Holm, K Otterdal, CP Dahl, K Krohg-Søre, EL Sagen, V Bjerkeli, AH Aamodt, A Abbas, I Gregersen, P Aukrust, B Halvorsen, TB Dahl
J Am Heart Assoc, 2018-01-12;7(2):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Lectin-type oxidized LDL receptor-1 distinguishes population of human polymorphonuclear myeloid-derived suppressor cells in cancer patients
Authors: T Condamine, GA Dominguez, JI Youn, AV Kossenkov, S Mony, K Alicea-Tor, E Tcyganov, A Hashimoto, Y Nefedova, C Lin, S Partlova, A Garfall, DT Vogl, X Xu, SC Knight, G Malietzis, GH Lee, E Eruslanov, SM Albelda, X Wang, JL Mehta, M Bewtra, A Rustgi, N Hockstein, R Witt, G Masters, B Nam, D Smirnov, MA Sepulveda, DI Gabrilovic
Sci Immunol, 2016-08-05;1(2):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Expression profile of cord blood neutrophils and dysregulation of HSPA1A and OLR1 upon challenge by bacterial peptidoglycan.
Authors: Fong O, Chan K, Leung K, Lam H, Cheung H, Leung T, Li K, Ng P
J Leukoc Biol, 2013-08-28;95(1):169-78.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Altered sciatic nerve fiber morphology and endoneural microvessels in mouse models relevant for obesity, peripheral diabetic polyneuropathy, and the metabolic syndrome.
Authors: Nowicki M, Kosacka J, Serke H, Bluher M, Spanel-Borowski K
J. Neurosci. Res., 2011-09-14;90(1):122-31.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot -
Traditional Chinese medication Tongxinluo dose-dependently enhances stability of vulnerable plaques: a comparison with a high-dose simvastatin therapy.
Authors: Zhang L, Liu Y, Lu XT, Wu YL, Zhang C, Ji XP, Wang R, Liu CX, Feng JB, Jiang H, Xu XS, Zhao YX, Zhang Y
Am. J. Physiol. Heart Circ. Physiol., 2009-10-02;297(6):H2004-14.
Species: Rabbit
Sample Types: Whole Cells
Applications: ICC -
The variable expression of lectin-like oxidized low-density lipoprotein receptor (LOX-1) and signs of autophagy and apoptosis in freshly harvested human granulosa cells depend on gonadotropin dose, age, and body weight.
Authors: Vilser C, Hueller H, Nowicki M, Hmeidan FA, Blumenauer V, Spanel-Borowski K
Fertil. Steril., 2009-04-01;93(8):2706-15.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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