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Human LPAR4/LPA4 Antibody

Catalog #: MAB10217 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10217-100
MAB10217-SP
Detection of LPAR4 in HEK293 Human Cell Line Transfected with Human LPAR4 and eGFP by Flow Cytometry.
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Human LPAR4/LPA4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LPAR4/LPA4 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 561203
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived transfected with human LPAR4/LPA4
Accession # Q99677
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LPAR4 antibody in HEK293 Human Cell Line Transfected with Human LPAR4 and eGFP antibody by Flow Cytometry. View Larger

Detection of LPAR4 in HEK293 Human Cell Line Transfected with Human LPAR4 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) Human LPAR4 or (B) irrelevant protein, and EGFP, was stained with Mouse Anti-Human LPAR4 Monoclonal Antibody (Catalog # MAB10217) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B isotype control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LPAR4/LPA4

Lysophosphatidic acid receptor 4, also known as LPA4, P2Y purinoceptor 9 (P2RY9) or GPR23, is a protein that in humans is encoded by the LPAR4 gene. LPA4 is a G protein-coupled receptor (GPCR) that binds the lipid signaling molecule lysophosphatidic acid (LPA) and mediates diverse cellular activities. Most LPA receptors share similarities with members of the S1P1/Edg family. GPA4, originally named P2Y9/GPR23, has been described as a fourth LPA receptor, LPA4, that together with LPA5 and LPA6 form a subfamily of LPA receptors structurally distant from the Edg family.  

Long Name
Lysophosphatidic Acid Receptor 4
Entrez Gene IDs
2846 (Human); 78134 (Mouse)
Alternate Names
G protein-coupled receptor 23; GPCR23; GPR23; GPR23P2Y purinoceptor 9; LPA4LPA-4; LPAR4; lysophosphatidic acid receptor 4; P2RY9; P2RY9G-protein coupled receptor 23; P2Y5-like receptor; P2Y5-LIKE; P2Y9; P2Y9LPA receptor 4; Purinergic receptor 9

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