Human LPAR5/LPA₅/GPR92 Antibody

Detection of LPAR5/LPA5/GPR92 in HEK293 Human Cell Line Transfected with Human LPAR5/LPA5/GPR92 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human LPAR5/LPA5/GPR92 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LPAR5/LPA5/GPR92 Monoclonal Antibody (Catalog # MAB10297) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

LPAR5/LPA₅/GPR92 in HT‑29 Human Cell Line. LPAR5/LPA₅/GPR92 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line treated with sodium butyrate (positive stain) and untreated (negative stain) using Mouse Anti-Human LPAR5/LPA₅/GPR92 Monoclonal Antibody (Catalog # MAB10297) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

LPAR5/LPA₅/GPR92 in Human Colon. LPAR5/LPA₅/GPR92 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human LPAR5/LPA₅/GPR92 Monoclonal Antibody (Catalog # MAB10297) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Goblet cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.