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Human LRIG2 Antibody

Catalog #: MAB1941 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1941-100
MAB1941-SP
Detection of LRIG-2 in HEK293 Human Cell Line Transfected with Human LRIG-2 and eGFP by Flow Cytometry.
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Human LRIG2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LRIG-2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 990310
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human LRIG-2
Gly41-Thr805
Accession # O94898
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LRIG-2 antibody in HEK293 Human Cell Line Transfected with Human LRIG-2 and eGFP antibody by Flow Cytometry. View Larger

Detection of LRIG-2 in HEK293 Human Cell Line Transfected with Human LRIG-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human LRIG-2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LRIG-2 Monoclonal Antibody (Catalog # MAB1941) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry LRIG2 antibody in WM-115 Human Cell Line by Immunocytochemistry (ICC). View Larger

LRIG2 in WM‑115 Human Cell Line. LRIG2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB1941) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry LRIG2 antibody in IMCD3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

LRIG2 in IMCD3 Mouse Cell Line. LRIG2 was detected in immersion fixed IMCD3 mouse inner medullary collecting duct cell line using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB1941) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRIG2

LRIG2 (leucine-rich repeats and Ig-like domains-2; also LIG-2) is an approximately 119-132 kDa glycoprotein that belongs to the LRIG gene family. The LRIG2 protein was predicted to have the same domain organization as LRIG1with a signal peptide, an extracellular part containing 15 leucine-rich repeats and three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tail. Over aa 41‑805, human LRIG2 shares 94%, 93% and 92% aa sequence identity with mouse, rat and macaque LRIG2 respectively.

Long Name
Leucine-rich Repeats and Immunoglobulin-like Domains 2
Entrez Gene IDs
9860 (Human); 269473 (Mouse); 310753 (Rat)
Alternate Names
KIAA0806; leucine-rich repeats and immunoglobulin-like domains 2; leucine-rich repeats and immunoglobulin-like domains protein 2; LIG2; LRIG2; UFS2

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