Human LRP-5 Intracellular Domain Antibody

Catalog #: MAB6646 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6646
MAB6646-SP

Detection of Human LRP-5 by Western Blot.

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All LRP-5 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LRP-5 Intracellular Domain Antibody Summary

Species Reactivity

Human

Specificity

Detects human LRP-5 Intracellular Domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with the intracellular domain of recombinant human LRP-6 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 747619

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human LRP-5 Intracellular Domain
Phe1422-Ser1615
Accession # O75197

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human LRP-5 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRP-5 Intracellular Domain Monoclonal Antibody (Catalog # MAB6646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRP-5 at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-5

LRP-5 (low-density lipoprotein receptor-related protein 5) is a 1615 amino acid (aa), ~180‑200 kDa type I transmembrane glycoprotein. The 1353 aa extracellular domain (ECD) contains 20 class B LDLR, 4 EGF‑like, and 3 class A LDLR repeats. Human LRP-5 aa 745-1097 encompasses class B LDLR repeats #12-17 and EGF‑like repeat #3. It shares 94% aa identity with mouse and rat LRP-5, and 70% aa identity with human LRP-6 within corresponding regions. LRP-5 and LRP-6 mediate DKK effects on Wnt signaling. LRP-5 polymorphisms correlate with susceptibility and resistance to osteoporosis.

Long Name

LDL Receptor-related Protein 5

Entrez Gene IDs

4041 (Human); 16973 (Mouse); 293649 (Rat)

Alternate Names

BMND1; BMND1OPTA1; EVR1; EVR4; HBM; low density lipoprotein receptor-related protein 5; low density lipoprotein receptor-related protein 7; low-density lipoprotein receptor-related protein 5; LR3VBCH2; LRP5; LRP-5; LRP7; LRP7exudative vitreoretinopathy 1; OPPG; OPS; OPTA1; osteoporosis pseudoglioma syndrome; VBCH2