Human LRP-6 PE-conjugated Antibody

Catalog #: FAB1505P Datasheet / COA / SDS

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FAB1505P

Detection of LRP‑6 in MDA‑MB‑231 Human Cell Line by Flow Cytometry.

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All LRP-6 Antibodies

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Human LRP-6 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human LRP-6 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) LRP-6 and no cross-reactivity with recombinant human (rh) LRP-1, -4, -5, rhLRP-6 (intracellular domain), or rhLDL R is observed.

Source

Monoclonal Mouse IgG_2A Clone # 255302

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LRP-6
Ala20-Pro1368
Accession # O75581

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LRP‑6 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human LRP-6 PE-conjugated Monoclonal Antibody (Catalog # FAB1505P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LRP-6

LRP-6 (Low-density lipoprotein receptor-related protein 6) is a 200-210 kDa member of the LDLR family of proteins. It is widely expressed, and serves as a coreceptor for both Wnt and parathyroid hormone. In the Wnt system, LRP-6 associates with select Fzd multipass receptors; in the PTH system, LRP-6 complexes with PTH1R. Mature human LRP-6 is a 1594 amino acid (aa) type I transmembrane glycoprotein. It contains a 1351 aa extracellular region (aa 20-1370) plus a 220 aa cytoplasmic domain (aa 1394-1613). The cytoplasmic domain contains two palmitoylation sites, one ubiquitination residue, and multiple phosphorylation motifs.

Long Name

LDL Receptor-related Protein 6

Entrez Gene IDs

4040 (Human); 16974 (Mouse)

Alternate Names

ADCAD2; FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP6; LRP-6