Human LRRC15 Antibody Summary
Met1-Gly538
Accession # Q8TF66
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of LRRC15 in HEK293 cells by Flow Cytometry. HEK293 cells transfected with Human LRRC-15 and eGFP were stained with eGFP and either (A) Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.
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Detection of Human LRRC15 by Simple WesternTM. Simple Western lane view shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LRRC15 at approximately 104 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human LRRC15 by Western Blot. Western blot shows lysates of Human skin and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LRRC15 at approximately ~70-80kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRRC15
LRRC15 is a type-I 15-leucine-rich repeat containing membrane protein that is located on chromosome 3 at 3q29. It is a 581 aa membrane protein that belong to the LRR superfamily and lacks an intracellular signaling domain. Reports indicate that it is highly expressed on cancer-associated fibroblasts in numerous solid tumors, and in the epithelial cancer cell compartment of glioblastoma, sarcomas and melanoma tumors. LRRC15 functions by regulating cell-cell and cell-extracellular matrix (ECM) interactions, likely partnering with fibronectin, laminin and collagen IV through its extracellular leucine-rich repeats. LRRC15 is reported in multiple solid tumor types including breast, ovarian and cervical cancers where it exhibits Increased stromal expression (1). Recent studies indicate that LRRC15 is co-expressed with COL10A1, a gene that is overexpressed in many types of solid tumors, including breast cancer (2).
- Cancer Res. 2022 March 15; 82(6): 1038. doi:10.1158/0008-5472.CAN-21-0622.
- Biosci Rep. 2020 Feb 28; 40(2): BSR20193286. doi: 10.1042/BSR20193286.
Product Datasheets
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