Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all LRRTM4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LRRTM4
Gln31-Lys424
Accession # Q86VH4

Specificity

Detects human LRRTM4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) LRRTM1, rhLRRTM2, and rhLRRTM3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human LRRTM4 Antibody

Detection of Human LRRTM4 antibody by Western Blot.

Detection of Human LRRTM4 by Western Blot.

Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LRRTM4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5377) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for LRRTM4 at approximately 65 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human LRRTM4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human brain tissue

Reviewed Applications

Read 1 review rated 4 using AF5377 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRRTM4

LRRTM4 (leucine rich repeat transmembrane protein 4) is a 59kDa (predicted) and 67 kDa (predicted) glycoprotein, member of the LRRTM family of proteins. All members of this family are type I transmembrane proteins that contain 10 external LRR repeats plus a short cytoplasmic tail that binds PDF motifs. LRRTM4 is found in the neurons of the cerebral cortex, dentate gyrus granule cells, and cerebellar Purkinje cells. Mature human LRRTM4 is 560 amino acids (aa) in length. The extracellular region contains one Cys‑rich N-terminal flanking domain (aa 33‑59), multiple LRRs (aa 60‑299), and a Cys‑rich C-terminal flanking domain (aa 306‑363). There is one splice variant that shows a Val substitution for cytoplasmic aa 518‑590. Over aa 31‑424, human LRRTM4 shares 95% aa identity with mouse LRRTM4.

Long Name

Leucine Rich Repeat Transmembrane Neuronal 4

Alternate Names

FLJ12568, leucine rich repeat transmembrane neuronal 4, leucine-rich repeat transmembrane neuronal 4 protein, leucine-rich repeat transmembrane neuronal protein 4, MGC120633, MGC120636

Entrez Gene IDs

80059 (Human); 500219 (Rat)

Gene Symbol

LRRTM4

UniProt

Q86VH4

Additional LRRTM4 Products

Product Documents for Human LRRTM4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LRRTM4 Antibody

For research use only

Related Research Areas

Activin Family

Citations for Human LRRTM4 Antibody

Customer Reviews for Human LRRTM4 Antibody (1)

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  • Human LRRTM4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: C57BL/6 mouse retina
    Species: Mouse
    Verified Customer | Posted 07/14/2020
    Whole mouse eyes were fixed for 10 min in 2% PFA, cryoprotected in 30% sucrose in PBS, and embedded in OCT. Sections were immunostained with LRRTM4 antibody, 1:40. Longer fixation times were detrimental to LRRTM4 detection.
    Human LRRTM4 Antibody AF5377

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