Human Lymphotoxin Antibody

Catalog #: MAB1370
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1370
MAB1370-SP

Neutralization of Lymphotoxin alpha 2/ beta 1 Bioactivity by Human Lymphotoxin Antibody

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All Lymphotoxin Antibodies

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Citations (3)

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Human Lymphotoxin Antibody Summary

Species Reactivity

Human

Specificity

Recognizes human Lymphotoxin alpha chains in alpha ₂ beta ₁ or alpha ₁ beta ₂ trimers and also human TNF-beta. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhFas Ligand, rhLIGHT, rhGITR Ligand, rhOX40 Ligand, recombinant mouse RANKL, rhTNF-alpha, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.

Source

Monoclonal Mouse IgG₁ Clone # 135125

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lymphotoxin alpha / beta

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize Lymphotoxin alpha 2/ beta 1induced cytotoxicity in the L929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.150 - 3.00 µg/mL in the presence of 10 ng/mL Recombinant Human Lymphotoxin alpha 2/ beta 1and actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Neutralization of Lymphotoxin alpha 2/ beta 1 Bioactivity by Human Lymphotoxin Antibody Mouse Anti-Human Lymphotoxin Antibody Clone # 135125 (Catalog # MAB1370) neutralizes Recombinant Human Lymphotoxin alpha 2/ beta 1 (679-TX) induced cytotoxicity in the L-929 mouse fibroblast cell line in the presence of actinomycin D. The ND₅₀ for this effect is typically 0.150-3.00 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin

Lymphotoxin alpha, also known as tumor necrosis factor (TNF) beta, and Lymphotoxin beta are members of the TNF superfamily and have been designated TNFSF1 and TNFSF3, respectively. Secreted Lymphotoxin alpha forms a homotrimer (TNFbeta) and heterotrimeric complexes with the type II membrane protein, Lymphotoxin beta (either alpha 2 beta 1or alpha 1 beta 2 timers). These trimers serve as ligands for Lymphotoxin beta receptor, TNF RI and TNF RII and play a role in the development and maturation of the immune system.

References

Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach.
Clemens, M. J. et al. (eds): IRL Press. 221.

Entrez Gene IDs

4049 (Human); 16992 (Mouse); 102138497 (Cynomolgus Monkey)

Alternate Names

LT; Lymphotoxin alpha; Lymphotoxin; tnfb; TNFSF1; TNLG1E

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Citations for Human Lymphotoxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Loss of the oncogenic phosphatase PRL-3 promotes a TNF-R1 feedback loop that mediates triple-negative breast cancer growth
Authors: H H Gari, G D DeGala, M S Lucia, J R Lambert
Oncogenesis
Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells.
Authors: Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra C, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Arostegui J, Juan M, Yague J, Merad M, Fagarasan S, Cerutti A
Nat Immunol, 2014-02-23;15(4):354-64.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
A role for lymphotoxin in primary Sjogren's disease.
Authors: Shen L, Suresh L, Wu J, Xuan J, Li H, Zhang C, Pankewycz O, Ambrus JL
J. Immunol., 2010-10-15;185(10):6355-63.
Species: Human, Mouse
Sample Types: Saliva
Applications: Western Blot