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Human MafG Antibody

Catalog #: MAB3924
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3924
MAB3924-SP
Detection of Human MafG by Western Blot.
2 Images
Product Details
Citations (1)
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Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MafG Antibody Summary

Species Reactivity
Human
Specificity
Detects human MafG in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MafF or rhMafK is observed.
Source
Monoclonal Mouse IgG2A Clone # 370412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MafG
Met1-Ser162
Accession # O15525
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MafG antibody by Western Blot. View Larger

Detection of Human MafG by Western Blot. Western blot shows recombinant human MafF, MafG, and MafK (5 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human MafG Monoclonal Antibody (Catalog # MAB3924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for MafG was detected at approximately 18 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human MafG antibody by Western Blot. View Larger

Detection of Human MafG by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human MafG Monoclonal Antibody (Catalog # MAB3924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for MafG was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MafG

Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members.

Long Name
v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog G
Entrez Gene IDs
4097 (Human); 17134 (Mouse); 64188 (Rat)
Alternate Names
basic leucine zipper transcription factor MafG; hMAF; MafG; MGC13090; MGC20149; transcription factor MafG; v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein G; v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian); V-maf musculoaponeurotic fibrosarcoma oncogene homolog G

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Citation for Human MafG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. DNA methylation instability by BRAF-mediated TET silencing and lifestyle-exposure divides colon cancer pathways
    Authors: Faiza Noreen, Taya Küng, Luigi Tornillo, Hannah Parker, Miguel Silva, Stefan Weis et al.
    Clinical Epigenetics

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