Human Magnetic Luminex® Performance Assay Base Kit A
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Build NowHuman Magnetic Luminex® Performance Assay Base Kit A Summary
Panel | Luminex Performance Human Cytokine Panel A |
Assay Type | Magnetic bead-based multiplex assay for the Luminex® platform |
Format | 1 x 96-well microplate and Magnetic antibody-coated beads |
Species | Human |
Analytes Detected | Please see analyte list in assay customization tool below. |
Performance Validation | Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet. |
Make your selections below to configure your assay
Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.
Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile up to analytes of your choice in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.
- User-mix antibody-coated Magnetic beads in individual vials
- User-mixed cocktail of biotinylated detection antibody
Precision
Intra-Assay Precision - is generated from the mean of the %CV's from 20 reportable results across three different concentrations of analytes in a single serum assay. Inter-Assay Precision - is generated from the mean of the %CV's across three different concentrations of analytes across 25 different serum assays. |
||
n=60 (20x3 levels) | n=25 | |
---|---|---|
Analyte | Intra-Assay (%CV) | Inter-Assay (%CV) |
CCL2/JE/MCP-1 | 5.3 | 7.8 |
CCL3/MIP-1 alpha | 12.5 | 11.8 |
CCL4/MIP-1 beta | 4.9 | 9.6 |
CCL5/RANTES | 9.5 | 11 |
CXCL5/ENA-78 | 4 | 9.9 |
FGF basic/FGF2/bFGF | 4.3 | 12.8 |
G-CSF | 4.8 | 9.4 |
GM-CSF | 4.4 | 11.4 |
IFN-gamma | 5.7 | 7.6 |
IL-1 alpha/IL-1F1 | 6.3 | 11.5 |
IL-1 beta/IL-1F2 | 5 | 9.1 |
IL-1ra/IL-1F3 | 3.7 | 8 |
IL-2 | 4.5 | 11.9 |
IL-4 | 3.5 | 12.1 |
IL-5 | 5.4 | 7.3 |
IL-6 | 4.5 | 6.8 |
IL-8/CXCL8 | 6 | 14 |
IL-10 | 5.6 | 8.3 |
IL-17/IL-17A | 4.7 | 7.9 |
TNF-alpha | 4.2 | 6.8 |
Thrombopoietin/Tpo | 5.5 | 7 |
VEGF | 5.8 | 8.6 |
Accuracy
Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples. |
||
Linearity | Spiked Recovery |
|
---|---|---|
Analyte | Average % | Average % |
CCL2/JE/MCP-1 | 134 | 102 |
CCL3/MIP-1 alpha | 98 | 106 |
CCL4/MIP-1 beta | 105 | 108 |
CCL5/RANTES | 104 | NO |
CXCL5/ENA-78 | 100 | 97 |
FGF basic/FGF2/bFGF | 91 | 95 |
G-CSF | 110 | 102 |
GM-CSF | 92 | 92 |
IFN-gamma | 102 | 103 |
IL-1 alpha/IL-1F1 | 108 | 86 |
IL-1 beta/IL-1F2 | 101 | 109 |
IL-1ra/IL-1F3 | 102 | 114 |
IL-2 | 99 | 109 |
IL-4 | 96 | 103 |
IL-5 | 98 | 106 |
IL-6 | 111 | 108 |
IL-8/CXCL8 | 102 | 112 |
IL-10 | 95 | 109 |
IL-17/IL-17A | 102 | 105 |
TNF-alpha | 107 | 108 |
Thrombopoietin/Tpo | 108 | 129 |
VEGF | 101 | 109 |
Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.
Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.
Product Datasheets
Citations for Human Magnetic Luminex® Performance Assay Base Kit A
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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EGR1 regulates oral epithelial cell responses to Candida albicans via the EGFR- ERK1/2 pathway
Authors: RE Dickenson, A Pellon, NO Ponde, O Hepworth, LF Daniels Ga, JR Naglik, DL Moyes
bioRxiv : the preprint server for biology, 2023-04-03;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Plasma Interleukin-6 Level Predicts the Risk of Arteriovenous Fistula Dysfunction in Patients Undergoing Maintenance Hemodialysis
Authors: J Baek, H Lee, T Yang, SY Lee, YG Kim, JS Kim, S Ahn, K Kim, SH Kang, MJ Lee, DY Lee, HY Jeong, YH Lee
Journal of personalized medicine, 2023-01-12;13(1):.
Species: Human
Sample Types: Plasma
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Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system
Authors: A Weigert, X Zheng, A Nenzel, K Turkowski, S Günther, E Strack, E Sirait-Fis, E Elwakeel, IM Kur, VS Nikam, C Valasaraja, H Winter, A Wissgott, R Voswinkel, F Grimminger, B Brüne, W Seeger, SS Pullamsett, R Savai
Nature Communications, 2022-10-14;13(1):6078.
Species: Human
Sample Types: Cell Culture Supernates
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Comprehensive Study of Antiretroviral Drug Permeability at the Cervicovaginal Mucosa via an In Vitro Model
Authors: C Carserides, K Smith, M Zinicola, A Kumar, M Swedrowska, C Scala, G Cameron, Z Riches, F Iannelli, G Pozzi, GL Hold, B Forbes, C Kelly, K Hijazi
Pharmaceutics, 2022-09-13;14(9):.
Species: Human
Sample Types: Cell Culture Supernates
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Serum Markers Associated with Disease Severity in a Bosnian Hemorrhagic Fever with Renal Syndrome Cohort
Authors: D Noack, M Travar, V Mrdjen, JJC Voermans, D van de Vij, R Molenkamp, MPG Koopmans, M Goeijenbie, B Rockx
Viruses, 2022-06-24;14(7):.
Species: Human
Sample Types: Serum
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Human Osteochondral Explants as an Ex Vivo Model of Osteoarthritis for the Assessment of a Novel Class of Orthobiologics
Authors: C Giannasi, L Mangiavini, S Niada, A Colombo, E Della Mort, V Vismara, A Ambrosanio, P Savadori, S Casati, GM Peretti, AT Brini
Pharmaceutics, 2022-06-10;14(6):.
Species: Human
Sample Types: Cell Culture Supernates
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Differential Inflammatory Responses in Cultured Endothelial Cells Exposed to Two Conjugated Linoleic Acids (CLAs) under a Pro-Inflammatory Condition
Authors: CA Valenzuela, EJ Baker, EA Miles, PC Calder
International Journal of Molecular Sciences, 2022-05-29;23(11):.
Species: Human
Sample Types: Cell Culture Supernates
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beta(2?6)-Type fructans attenuate proinflammatory responses in a structure dependent fashion via Toll-like receptors
Authors: C Fernández-, R Akkerman, MMP Oerlemans, MJ Logtenberg, HA Schols, LA Silva-Lago, G López-Velá, P de Vos
Carbohydrate polymers, 2021-11-15;277(0):118893.
Species: Human
Sample Types: Cell Culture Supernates
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Clinical and laboratory characteristics of hemophagocytic lymphohistiocytosis induced by Leishmania infantum infection
Authors: Q Shi, M Huang, X Li, X Zheng, F Wang, Y Zou, L Wang, J Jia
PloS Neglected Tropical Diseases, 2021-11-04;15(11):e0009944.
Species: Human
Sample Types: Serum
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Host characteristics associated with serologic inflammatory biomarkers in women
Authors: SS Wang, C Zhong, M Epeldegui, S Nunes, L Magpantay, JC DeHart, S Hurley, D Goldberg, E Martinez, JV Lacey, O Martinez-M, P Reynolds
Cytokine, 2021-10-16;149(0):155726.
Species: Human
Sample Types: Serum
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Modeling SARS-CoV-2 and Influenza Infections and Antiviral Treatments in Human Lung Epithelial Tissue Equivalents
Authors: H Zarkoob, A Allué-Guar, YC Chen, O Jung, A Garcia-Vil, MJ Song, JG Park, F Oladunni, J Miller, YT Tung, I Kosik, D Schultz, J Yewdell, JB Torrelles, L Martinez-S, S Cherry, M Ferrer, EM Lee
bioRxiv : the preprint server for biology, 2021-05-12;0(0):.
Species: Human
Sample Types: BALF
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Association between Low Muscle Mass and Inflammatory Cytokines
Authors: S Ito, H Nakashima, K Ando, K Kobayashi, M Machino, T Seki, S Ishizuka, R Fujii, Y Takegami, H Yamada, Y Ando, K Suzuki, Y Hasegawa, S Imagama
BioMed Research International, 2021-04-27;2021(0):5572742.
Species: Human
Sample Types: Serum
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IKKepsilon and TBK1 in diffuse large B-cell lymphoma: A possible mechanism of action of an IKKepsilon/TBK1 inhibitor to repress NF-kappaB and IL-10 signalling
Authors: M Carr, S Mamand, KL Chapman, T Perrior, SD Wagner
J. Cell. Mol. Med., 2020-08-28;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Correlation of host inflammatory cytokines and immune-related metabolites, but not viral NS1 protein, with disease severity of dengue virus infection
Authors: HJ Soe, R Manikam, CS Raju, MA Khan, SD Sekaran
PLoS ONE, 2020-08-07;15(8):e0237141.
Species: Human
Sample Types: Serum
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Repeated Application of Autologous Bone Marrow-Derived Lineage-Negative Stem/Progenitor Cells-Focus on Immunological Pathways in Patients with ALS
Authors: B Baumert, A Sobu?, M Go??b-Jano, E Paczkowska, K ?uczkowska, D Rogi?ska, A Zawi?lak, S Milczarek, B Os?kowska, W Pawlukowsk, A Meller, K Machowska-, A We?nicka, K Safranow, P Nowacki, B Machali?sk
Cells, 2020-08-01;9(8):.
Species: Human
Sample Types: Plasma
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Guanine Deaminase in Human Epidermal Keratinocytes Contributes to Skin Pigmentation
Authors: JM Jung, TK Noh, SY Jo, SY Kim, Y Song, YH Kim, SE Chang
Molecules, 2020-06-05;25(11):.
Species: Human
Sample Types: Serum
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Targeting tumor-resident mast cells for effective anti-melanoma immune responses
Authors: S Kaesler, F Wölbing, WE Kempf, Y Skabytska, M Köberle, T Volz, T Sinnberg, T Amaral, S Möckel, A Yazdi, G Metzler, M Schaller, K Hartmann, B Weide, C Garbe, HG Rammensee, M Röcken, T Biedermann
JCI Insight, 2019-10-03;4(19):.
Species: Human
Sample Types: Serum
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Candidalysin activates innate epithelial immune responses via epidermal growth factor receptor
Authors: J Ho, X Yang, SA Nikou, N Kichik, A Donkin, NO Ponde, JP Richardson, RL Gratacap, LS Archambaul, CP Zwirner, C Murciano, R Henley-Smi, S Thavaraj, CJ Tynan, SL Gaffen, B Hube, RT Wheeler, DL Moyes, JR Naglik
Nat Commun, 2019-05-24;10(1):2297.
Species: Human
Sample Types: Cell Culture Supernates
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Patients with idiopathic recurrent miscarriage have abnormally high TGF�+ blood NK, NKT and T cells in the presence of abnormally low TGF� plasma levels
Authors: L Zhu, M Aly, RJ Kuon, B Toth, H Wang, H Karakizlis, R Weimer, C Morath, E Ibrahim, N Ekpoom, G Opelz, V Daniel
BMC Immunol., 2019-03-04;20(1):10.
Species: Human
Sample Types: Plasma
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A ferroptosis-based panel of prognostic biomarkers for Amyotrophic Lateral Sclerosis
Authors: D Devos, C Moreau, M Kyheng, G Garçon, AS Rolland, H Blasco, P Gelé, T Timothée L, C Veyrat-Dur, P Corcia, M Dutheil, P Bede, A Jeromin, P Oeckl, M Otto, V Meninger, V Danel-Brun, JC Devedjian, JA Duce, PF Pradat
Sci Rep, 2019-02-27;9(1):2918.
Species: Human
Sample Types: Plasma
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Evasion of Immune Surveillance in Low Oxygen Environments Enhances Candida albicans Virulence
Authors: JP Lopes, M Stylianou, E Backman, S Holmberg, J Jass, R Claesson, CF Urban
MBio, 2018-11-06;9(6):.
Species: Human
Sample Types: Cell Culture Supernates
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Effect of colorectal cancer-derived extracellular vesicles on the immunophenotype and cytokine secretion profile of monocytes and macrophages
Authors: I Pop?na, A ?bols, L Saul?te, K Pleiko, E Zandberga, K J?kabsons, E Endzeli?š, A Llorente, A Lin?, U Rieksti?a
Cell Commun. Signal, 2018-04-24;16(1):17.
Species: Human
Sample Types: Cell Culture Supernates
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A randomised study in young subjects of the effects of eating extra fruit or nuts on periodontal inflammation
BDJ Open, 2018-02-09;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Processing of Candida albicans Ece1p Is Critical for Candidalysin Maturation and Fungal Virulence
Authors: JP Richardson, S Mogavero, DL Moyes, M Blagojevic, T Krüger, AH Verma, BM Coleman, J De La Cruz, D Schulz, NO Ponde, G Carrano, O Kniemeyer, D Wilson, O Bader, SI Enoiu, J Ho, N Kichik, SL Gaffen, B Hube, JR Naglik
MBio, 2018-01-23;9(1):.
Species: Human
Sample Types: Cell Culture Supernates
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Annexin A1 in blood mononuclear cells from patients with coronary artery disease: Its association with inflammatory status and glucocorticoid sensitivity
Authors: I Bergström, AK Lundberg, S Jönsson, E Särndahl, J Ernerudh, L Jonasson
PLoS ONE, 2017-03-22;12(3):e0174177.
Species: Human
Sample Types: Cell Culture Supernates
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Immunomodulatory Properties of 1,2-Dihydro-4-hydroxy-2-oxo-1,8-naphthyridine-3-carboxamide Derivative VL15
Authors: AM Malfitano, C Laezza, S Bertini, D Marasco, T Tuccinardi, M Bifulco, C Manera
Biochimie, 2017-02-20;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Elevated cerebral spinal fluid biomarkers in children with mucopolysaccharidosis I-H
Sci Rep, 2016-12-02;6(0):38305.
Species: Human
Sample Types: CSF
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Association of Mucosal Organisms with Patterns of Inflammation in Chronic Rhinosinusitis.
Authors: Chalermwatanachai T, Zhang N, Holtappels G, Bachert C
PLoS ONE, 2015-08-14;10(8):e0136068.
Species: Human
Sample Types: Tissue Homogenates
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Differential cytokine profiles upon comparing selective versus classic glucocorticoid receptor modulation in human peripheral blood mononuclear cells and inferior turbinate tissue.
Authors: Beck I, Van Crombruggen K, Holtappels G, Daubeuf F, Frossard N, Bachert C, De Bosscher K
PLoS ONE, 2015-04-13;10(4):e0123068.
Species: Human
Sample Types: Cell Culture Supernates
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A randomized trial of an early measles vaccine at 4(1/2) months of age in Guinea-Bissau: sex-differential immunological effects.
Authors: Jensen K, Sondergaard M, Andersen A, Sartono E, Martins C, Garly M, Eugen-Olsen J, Ullum H, Yazdanbakhsh M, Aaby P, Benn C, Erikstrup C
PLoS ONE, 2014-05-16;9(5):e97536.
Species: Human
Sample Types: Plasma
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Survey of innate immune responses to Burkholderia pseudomallei in human blood identifies a central role for lipopolysaccharide.
Authors: Chantratita N, Tandhavanant S, Myers N, Seal S, Arayawichanont A, Kliangsa-Ad A, Hittle L, Ernst R, Emond M, Wurfel M, Day N, Peacock S, West T
PLoS ONE, 2013-11-26;8(11):e81617.
Species: Human
Sample Types: Plasma
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N6-isopentenyladenosine, an endogenous isoprenoid end product, directly affects cytotoxic and regulatory functions of human NK cells through FDPS modulation.
Authors: Ciaglia E, Pisanti S, Picardi P, Laezza C, Malfitano A, D'Alessandro A, Gazzerro P, Vitale M, Carbone E, Bifulco M
J Leukoc Biol, 2013-07-11;94(6):1207-19.
Species: Human
Sample Types: Cell Culture Supernates
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Protein expression profiling of inflammatory mediators in human temporal lobe epilepsy reveals co-activation of multiple chemokines and cytokines.
Authors: Kan A, De Jager W, de Wit M, Heijnen C, van Zuiden M, Ferrier C, van Rijen P, Gosselaar P, Hessel E, van Nieuwenhuizen O, de Graan P
J Neuroinflammation, 2012-08-30;9(0):207.
Species: Human
Sample Types: Tissue Homogenates
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Role of toll like receptors in irritable bowel syndrome: differential mucosal immune activation according to the disease subtype.
Authors: Belmonte L, Beutheu Youmba S, Bertiaux-Vandaele N, Antonietti M, Lecleire S, Zalar A, Gourcerol G, Leroi A, Dechelotte P, Coeffier M, Ducrotte P
PLoS ONE, 2012-08-17;7(8):e42777.
Species: Human
Sample Types: Tissue Homogenates
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Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
J. Thromb. Haemost., 2012-08-01;10(8):1616-23.
Species: Human
Sample Types: Plasma
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REDD1 protects osteoblast cells from gamma radiation-induced premature senescence.
Authors: Li XH, Ha CT, Fu D, Xiao M
PLoS ONE, 2012-05-18;7(5):e36604.
Species: Human
Sample Types: Cell Culture Supernates
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The degree of leukocytosis and urine GATA-3 mRNA levels are risk factors for severe acute kidney injury in Puumala virus nephropathia epidemica.
Authors: Libraty DH, Makela S, Vlk J, Hurme M, Vaheri A, Ennis FA, Mustonen J
PLoS ONE, 2012-04-16;7(4):e35402.
Species: Human
Sample Types: Plasma
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Epithelial proinflammatory response and curcumin-mediated protection from staphylococcal toxic shock syndrome toxin-1.
Authors: Schaefers MM, Breshears LM, Anderson MJ, Lin YC, Grill AE, Panyam J, Southern PJ, Schlievert PM, Peterson ML
PLoS ONE, 2012-03-14;7(3):e32813.
Species: Porcine
Sample Types: Cell Culture Supernates
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Evaluation of the role of Candida albicans agglutinin-like sequence (Als) proteins in human oral epithelial cell interactions.
Authors: Murciano C, Moyes DL, Runglall M, Tobouti P, Islam A, Hoyer LL, Naglik JR
PLoS ONE, 2012-03-12;7(3):e33362.
Species: Human
Sample Types: Cell Culture Supernates
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Elevated cerebral spinal fluid cytokine levels in boys with cerebral adrenoleukodystrophy correlates with MRI severity.
Authors: Lund TC, Stadem PS, Panoskaltsis-Mortari A, Raymond G, Miller WP, Tolar J, Orchard PJ
PLoS ONE, 2012-02-16;7(2):e32218.
Species: Human
Sample Types: Serum
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Effects of methylprednisolone infusion on markers of inflammation, coagulation, and angiogenesis in early acute respiratory distress syndrome.
Authors: Seam N, Meduri GU, Wang H, Nylen ES, Sun J, Schultz MJ, Tropea M, Suffredini AF
Crit. Care Med., 2012-02-01;40(2):495-501.
Species: Human
Sample Types: Plasma
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RIP1 is required for IAP inhibitor-mediated sensitization of childhood acute leukemia cells to chemotherapy-induced apoptosis.
Authors: Loder S, Fakler M, Schoeneberger H, Cristofanon S, Leibacher J, Vanlangenakker N, Bertrand MJ, Vandenabeele P, Jeremias I, Debatin KM, Fulda S
Leukemia, 2011-12-16;26(5):1020-9.
Species: Human
Sample Types: Cell Culture Supernates
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Candida albicans yeast and hyphae are discriminated by MAPK signaling in vaginal epithelial cells.
Authors: Moyes DL, Murciano C, Runglall M, Islam A, Thavaraj S, Naglik JR
PLoS ONE, 2011-11-08;6(11):e26580.
Species: Human
Sample Types: Cell Culture Supernates
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Macrophage infiltration predicts a poor prognosis for human ewing sarcoma.
Authors: Fujiwara T, Fukushi J, Yamamoto S, Matsumoto Y, Setsu N, Oda Y, Yamada H, Okada S, Watari K, Ono M, Kuwano M, Kamura S, Iida K, Okada Y, Koga M, Iwamoto Y
Am. J. Pathol., 2011-07-21;179(3):1157-70.
Species: Human
Sample Types: Cell Culture Supernates
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Anti-Inflammatory Cytokines Predominate in Acute Human Plasmodium knowlesi Infections.
Authors: Cox-Singh J, Singh B, Daneshvar C, Planche T, Parker-Williams J, Krishna S
PLoS ONE, 2011-06-08;6(6):e20541.
Species: Human
Sample Types: Serum
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Effect of Lactobacillus paracasei ST11 on a nasal provocation test with grass pollen in allergic rhinitis.
Authors: Wassenberg J, Nutten S, Audran R, Barbier N, Aubert V, Moulin J, Mercenier A, Spertini F
Clin. Exp. Allergy, 2011-04-01;41(4):565-73.
Species: Human
Sample Types: Nasal Fluid
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Probiotic Lactobacillus casei Shirota supplementation does not modulate immunity in healthy men with reduced natural killer cell activity.
Authors: Seifert S, Bub A, Franz CM, Watzl B
J. Nutr., 2011-03-23;141(5):978-84.
Species: Human
Sample Types: Cell Culture Supernates
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The novel antisense Bcl-2 inhibitor SPC2996 causes rapid leukemic cell clearance and immune activation in chronic lymphocytic leukemia.
Authors: Durig J, Duhrsen U, Klein-Hitpass L, Worm J, Hansen JB, Orum H, Wissenbach M
Leukemia, 2011-03-01;25(4):638-47.
Species: Human
Sample Types: Serum
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Pro- and anti-inflammatory cytokines in latent autoimmune diabetes in adults, type 1 and type 2 diabetes patients: Action LADA 4.
Authors: Pham MN, Hawa MI, Pfleger C, Roden M, Schernthaner G, Pozzilli P, Buzzetti R, Scherbaum W, Seissler J, Kolb H, Hunter S, Leslie RD, Schloot NC
Diabetologia, 2011-02-24;54(7):1630-8.
Species: Human
Sample Types: Serum
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The IL23R R381Q Gene Variant Protects against Immune-Mediated Diseases by Impairing IL-23-Induced Th17 Effector Response in Humans.
Authors: Di Meglio P, Di Cesare A, Laggner U
PLoS ONE, 2011-02-22;6(2):e17160.
Species: Human
Sample Types: Cell Culture Supernates
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Lipid-heparin infusion suppresses the IL-10 response to trauma in subcutaneous adipose tissue in humans.
Authors: Ikeoka DT, Pachler C, Mader JK, Bock G, Neves AL, Svehlikova E, Feichtner F, Koehler G, Wrighton CJ, Pieber TR, Ellmerer M
Obesity (Silver Spring), 2010-11-18;19(4):715-21.
Species: Human
Sample Types: Complex Sample Type
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Potential biomarkers of osteonecrosis in Gaucher disease.
Authors: Pavlova EV, Deegan PB, Tindall J, McFarlane I, Mehta A, Hughes D, Wraith JE, Cox TM
Blood Cells Mol. Dis., 2010-11-13;46(1):27-33.
Species: Human
Sample Types: Serum
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Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells.
Authors: Ramirez JM, Brembilla NC, Sorg O, Chicheportiche R, Matthes T, Dayer JM, Saurat JH, Roosnek E, Chizzolini C
Eur. J. Immunol., 2010-09-01;40(9):2450-9.
Species: Human
Sample Types: Cell Culture Supernates
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Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells.
Authors: Liang X, Moseman EA, Farrar MA
Blood, 2010-03-25;115(24):5041-52.
Species: Human
Sample Types: Cell Culture Supernates
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Serum IL-6: a candidate biomarker for intracranial pressure elevation following isolated traumatic brain injury.
Authors: Hergenroeder GW, Moore AN, McCoy JP
J Neuroinflammation, 2010-03-11;7(0):19.
Species: Human
Sample Types: Serum
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Phase I/II study of the Src inhibitor dasatinib in combination with erlotinib in advanced non-small-cell lung cancer.
Authors: Haura EB, Tanvetyanon T, Chiappori A, Williams C, Simon G, Antonia S, Gray J, Litschauer S, Tetteh L, Neuger A, Song L, Rawal B, Schell MJ, Bepler G
J. Clin. Oncol., 2010-02-08;28(8):1387-94.
Species: Human
Sample Types: Plasma
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Remodeling of skeletal muscle microvasculature in sickle cell trait and alpha-thalassemia.
Authors: Vincent L, Feasson L, Oyono-Enguelle S, Banimbek V, Denis C, Guarneri C, Aufradet E, Monchanin G, Martin C, Gozal D, Dohbobga M, Wouassi D, Garet M, Thiriet P, Messonnier L
Am. J. Physiol. Heart Circ. Physiol., 2009-11-13;298(2):H375-84.
Species: Human
Sample Types: Plasma
-
Validity of multiplex-based assays for cytokine measurements in serum and plasma from "non-diseased" subjects: comparison with ELISA.
Authors: Dossus L, Becker S, Achaintre D, Kaaks R, Rinaldi S
J. Immunol. Methods, 2009-09-10;350(1):125-32.
Species: Human
Sample Types: Serum
-
The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells.
Authors: Schrauf C, Kirchberger S, Majdic O, Seyerl M, Zlabinger GJ, Stuhlmeier KM, Sachet M, Seipelt J, Stockl J
J. Immunol., 2009-09-04;183(7):4440-8.
Species: Human
Sample Types: Cell Culture Supernates
-
Homeostatic chemokines CCL19 and CCL21 promote inflammation in human immunodeficiency virus-infected patients with ongoing viral replication.
Authors: Damas JK, Landro L, Fevang B, Heggelund L, Tjonnfjord GE, Floisand Y, Halvorsen B, Froland SS, Aukrust P
Clin. Exp. Immunol., 2009-09-01;157(3):400-7.
Species: Human
Sample Types: Cell Culture Supernates
-
Endothelial dysfunction and specific inflammation in obesity hypoventilation syndrome.
Authors: Borel JC, Roux-Lombard P, Tamisier R, Arnaud C, Monneret D, Arnol N, Baguet JP, Levy P, Pepin JL
PLoS ONE, 2009-08-24;4(8):e6733.
Species: Human
Sample Types: Serum
-
Clinical efficacy and immune regulation with peanut oral immunotherapy.
Authors: Jones SM, Pons L, Roberts JL, Scurlock AM, Perry TT, Kulis M, Shreffler WG, Steele P, Henry KA, Adair M, Francis JM, Durham S, Vickery BP, Zhong X, Burks AW
J. Allergy Clin. Immunol., 2009-07-03;124(2):292-300, 300..
Species: Human
Sample Types: Cell Culture Supernates
-
Sputum desmosine during hospital admission for pulmonary exacerbation in cystic fibrosis.
Authors: Laguna TA, Wagner BD, Luckey HK, Mann SA, Sagel SD, Regelmann W, Accurso FJ
Chest, 2009-06-30;136(6):1561-8.
Species: Human
Sample Types: Sputum
-
Fatigue in primary Sj0gren's syndrome--a link to sickness behaviour in animals?
Authors: Harboe E, Tjensvoll AB, Vefring HK, Goransson LG, Kvaloy JT, Omdal R
Brain Behav. Immun., 2009-06-26;23(8):1104-8.
Species: Human
Sample Types: CSF
-
Neisseria gonorrhoeae activates the proteinase cathepsin B to mediate the signaling activities of the NLRP3 and ASC-containing inflammasome.
Authors: Duncan JA, Gao X, Huang MT, O'Connor BP, Thomas CE, Willingham SB, Bergstralh DT, Jarvis GA, Sparling PF, Ting JP
J. Immunol., 2009-05-15;182(10):6460-9.
Species: Human
Sample Types: Cell Culture Supernates
-
Effect of systemic inflammation on inspiratory and limb muscle strength and bulk in cystic fibrosis.
Authors: Dufresne V, Knoop C, Van Muylem A, Malfroot A, Lamotte M, Opdekamp C, Deboeck G, Cassart M, Stallenberg B, Casimir G, Duchateau J, Estenne M
Am. J. Respir. Crit. Care Med., 2009-04-02;180(2):153-8.
Species: Human
Sample Types: Serum
-
Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component.
Authors: O'Keefe BR, Vojdani F, Buffa V, Shattock RJ, Montefiori DC, Bakke J, Mirsalis J, d'Andrea AL, Hume SD, Bratcher B, Saucedo CJ, McMahon JB, Pogue GP, Palmer KE
Proc. Natl. Acad. Sci. U.S.A., 2009-03-30;106(15):6099-104.
Species: Human
Sample Types: Cell Culture Supernates
-
Simvastatin decreases lipopolysaccharide-induced pulmonary inflammation in healthy volunteers.
Authors: Shyamsundar M, McKeown ST, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, Craig TR, Brown V, Thickett DR, Matthay MA, Taggart CC, Backman JT, Elborn JS, McAuley DF
Am. J. Respir. Crit. Care Med., 2009-03-26;179(12):1107-14.
Species: Human
Sample Types: BALF
-
Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo.
Authors: Bendrik C, Dabrosin C
J. Immunol., 2009-01-01;182(1):371-8.
Species: Human
Sample Types: Plasma
-
Cigarette smoke facilitates allergen penetration across respiratory epithelium.
Authors: Gangl K, Reininger R, Bernhard D, Campana R, Pree I, Reisinger J, Kneidinger M, Kundi M, Dolznig H, Thurnher D, Valent P, Chen KW, Vrtala S, Spitzauer S, Valenta R, Niederberger V
Allergy, 2008-12-16;64(3):398-405.
Species: Human
Sample Types: Cell Culture Supernates
-
NF-kappaB modulates sensitivity to apoptosis, proinflammatory and migratory potential in short- versus long-term cultured human gammadelta lymphocytes.
Authors: Ferrarini M, Delfanti F, Gianolini M, Rizzi C, Alfano M, Lazzarin A, Biswas P
J. Immunol., 2008-11-01;181(9):5857-64.
Species: Human
Sample Types: Cell Culture Supernates
-
Critical and differential roles of NKp46- and NKp30-activating receptors expressed by uterine NK cells in early pregnancy.
Authors: El Costa H, Casemayou A, Aguerre-Girr M, Rabot M, Berrebi A, Parant O, Clouet-Delannoy M, Lombardelli L, Jabrane-Ferrat N, Rukavina D, Bensussan A, Piccinni MP, Le Bouteiller P, Tabiasco J
J. Immunol., 2008-09-01;181(0):3009-17.
Species: Human
Sample Types: Cell Culture Supernates
-
An evaluation of commercial fluorescent bead-based luminex cytokine assays.
Authors: Djoba Siawaya JF, Roberts T, Babb C, Black G, Golakai HJ, Stanley K, Bapela NB, Hoal E, Parida S, van Helden P, Walzl G
PLoS ONE, 2008-07-02;3(7):e2535.
Species: Human
Sample Types: Serum
-
Relation of circulating concentrations of chemokine receptor CCR5 ligands to C-peptide, proinsulin and HbA1c and disease progression in type 1 diabetes.
Authors: Pfleger C, Kaas A, Hansen L, Alizadeh B, Hougaard P, Holl R, Kolb H, Roep BO, Mortensen HB, Schloot NC
Clin. Immunol., 2008-04-22;128(1):57-65.
Species: Human
Sample Types: Serum
-
Association of IL-1ra and adiponectin with C-peptide and remission in patients with type 1 diabetes.
Authors: Pfleger C, Mortensen HB, Hansen L, Herder C, Roep BO, Hoey H, Aanstoot HJ, Kocova M, Schloot NC
Diabetes, 2008-02-25;57(4):929-37.
-
Sleeping Beauty transposon-mediated engineering of human primary T cells for therapy of CD19+ lymphoid malignancies.
Authors: Huang X, Guo H, Kang J, Choi S, Zhou TC, Tammana S, Lees CJ, Li ZZ, Milone M, Levine BL, Tolar J, June CH, Scott McIvor R, Wagner JE, Blazar BR, Zhou X
Mol. Ther., 2008-01-29;16(3):580-9.
-
Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection.
Authors: Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B
J. Virol., 2008-01-23;82(7):3736-50.
-
The IgE-reactive autoantigen Hom s 2 induces damage of respiratory epithelial cells and keratinocytes via induction of IFN-gamma.
Authors: Mittermann I, Reininger R, Zimmermann M, Gangl K, Reisinger J, Aichberger KJ, Greisenegger EK, Niederberger V, Seipelt J, Bohle B, Kopp T, Akdis CA, Spitzauer S, Valent P, Valenta R
J. Invest. Dermatol., 2007-12-13;128(6):1451-9.
Species: Human
Sample Types: Cell Culture Supernates
-
Defining cancer cachexia in head and neck squamous cell carcinoma.
Authors: Richey LM, George JR, Couch ME, Kanapkey BK, Yin X, Cannon T, Stewart PW, Weissler MC, Shores CG
Clin. Cancer Res., 2007-11-15;13(22):6561-7.
-
Potent induction of TNF-alpha during interaction of immune effectors with oral tumors as a potential mechanism for the loss of NK cell viability and function.
Authors: Romero-Reyes M, Head C, Cacalano NA, Jewett A
Apoptosis, 2007-11-01;12(11):2063-75.
-
Circulating levels of cytokines during pregnancy: thrombopoietin is elevated in miscarriage.
Authors: Whitcomb BW, Schisterman EF, Klebanoff MA, Baumgarten M, Luo X, Chegini N
Fertil. Steril., 2007-08-13;89(6):1795-802.
Species: Human
Sample Types: Serum
-
Pronounced elevation of resistin correlates with severity of disease in severe sepsis and septic shock.
Authors: Sunden-Cullberg J, Nystrom T, Lee ML, Mullins GE, Tokics L, Andersson J, Norrby-Teglund A, Treutiger CJ
Crit. Care Med., 2007-06-01;35(6):1536-42.
-
Circulating chemokine levels and miscarriage.
Authors: Whitcomb BW, Schisterman EF, Klebanoff MA, Baumgarten M, Rhoton-Vlasak A, Luo X, Chegini N
Am. J. Epidemiol., 2007-05-15;166(3):323-31.
-
Haemophilus influenzae induces neutrophil necrosis: a role in chronic obstructive pulmonary disease?
Authors: Naylor EJ, Bakstad D, Biffen M, Thong B, Calverley P, Scott S, Hart CA, Moots RJ, Edwards SW
Am. J. Respir. Cell Mol. Biol., 2007-03-15;37(2):135-43.
-
Inhibition of human cord blood-derived mast cell responses by anti-Fc epsilon RI mAb 15/1 versus anti-IgE Omalizumab.
Authors: Mirkina I, Schweighoffer T, Kricek F
Immunol. Lett., 2007-03-01;109(2):120-8.
-
Increased frequency of CD4+ cells expressing CD161 in cancer patients.
Authors: Iliopoulou EG, Karamouzis MV, Missitzis I, Ardavanis A, Sotiriadou NN, Baxevanis CN, Rigatos G, Papamichail M, Perez SA
Clin. Cancer Res., 2006-12-01;12(23):6901-9.
-
A neoadjuvant/adjuvant randomized trial of colorectal cancer patients vaccinated with an anti-idiotypic antibody, 105AD7, mimicking CD55.
Authors: Ullenhag GJ, Spendlove I, Watson NF, Indar AA, Dube M, Robins RA, Maxwell-Armstrong C, Scholefield JH, Durrant LG
Clin. Cancer Res., 2006-11-22;12(24):7389-96.
-
Peroxidated squalene induces the production of inflammatory mediators in HaCaT keratinocytes: a possible role in acne vulgaris.
Authors: Ottaviani M, Alestas T, Flori E, Mastrofrancesco A, Zouboulis CC, Picardo M
J. Invest. Dermatol., 2006-06-15;126(11):2430-7.
-
Biological material on inhaled coarse fraction particulate matter activates airway phagocytes in vivo in healthy volunteers.
Authors: Alexis NE, Lay JC, Zeman K, Bennett WE, Peden DB, Soukup JM, Devlin RB, Becker S
J. Allergy Clin. Immunol., 2006-04-27;117(6):1396-403.
Species: Human
Sample Types: Saliva
-
Coengagement of CD16 and CD94 receptors mediates secretion of chemokines and induces apoptotic death of naive natural killer cells.
Authors: Jewett A, Cacalano NA, Head C, Teruel A
Clin. Cancer Res., 2006-04-01;12(7):1994-2003.
-
Sensitization of IL-2 signaling through TLR-7 enhances B lymphoma cell immunogenicity.
Authors: Tomic J, White D, Shi Y, Mena J, Hammond C, He L, Miller RL, Spaner DE
J. Immunol., 2006-03-15;176(6):3830-9.
-
Positive correlation between estradiol and vascular endothelial growth factor but not fibroblast growth factor-2 in normal human breast tissue in vivo.
Authors: Dabrosin C
Clin. Cancer Res., 2005-11-15;11(22):8036-41.
-
Secretion of proinflammatory cytokines and chemokines during amphotericin B exposure is mediated by coactivation of toll-like receptors 1 and 2.
Authors: Razonable RR, Henault M, Lee LN, Laethem C, Johnston PA, Watson HL, Paya CV
Antimicrob. Agents Chemother., 2005-04-01;49(4):1617-21.
-
Anti-inflammatory effects of UV-irradiated lymphocytes: induction of IL-1Ra upon phagocytosis by monocyte/macrophages.
Authors: Craciun LI, DiGiambattista M, Schandene L, Laub R, Goldman M, Dupont E
Clin. Immunol., 2005-03-01;114(3):320-6.
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Human plasmacytoid dendritic cells activated by CpG oligodeoxynucleotides induce the generation of CD4+CD25+ regulatory T cells.
Authors: Moseman EA, Liang X, Dawson AJ, Panoskaltsis-Mortari A, Krieg AM, Liu YJ, Blazar BR, Chen W
J. Immunol., 2004-10-01;173(7):4433-42.
-
Multiplex bead array assays for detection of soluble cytokines: comparisons of sensitivity and quantitative values among kits from multiple manufacturers.
Authors: Khan SS, Smith MS, Reda D, Suffredini AF, McCoy JP
Cytometry B Clin Cytom, 2004-09-01;61(1):35-9.
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Exercise acutely increases circulating endothelial progenitor cells and monocyte-/macrophage-derived angiogenic cells.
Authors: Rehman J, Li J, Parvathaneni L, Karlsson G, Panchal VR, Temm CJ, Mahenthiran J, March KL
J. Am. Coll. Cardiol., 2004-06-16;43(12):2314-8.
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In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB
Blood, 2004-03-18;104(2):453-61.
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An in vitro tumor model: analysis of angiogenic factor expression after chemotherapy.
Authors: Keyes K, Cox K, Treadway P, Mann L, Shih C, Faul MM, Teicher BA
Cancer Res., 2002-10-01;62(19):5597-602.
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The assay worked as expected, with low % CV's