Human MANF Antibody Summary
Leu22-Leu179
Accession # P55145
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human MANF by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, U‑87 MG human glioblastoma/astrocytoma cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MANF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MANF at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Detection of Human MANF by Simple WesternTM. Simple Western lane view shows lysates of SH-SY5Y human neuroblastoma cell line and U-87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MANF at approximately 24 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MANF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3748) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western Blot Shows Human MANF Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and MANF knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MANF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MANF at approximately 17 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293Tcell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MANF
Mesencephalic astrocyte-derived neurotrophic factor (MANF), also known as arginine-rich, mutated in early stage tumors (ARMET) and arginine-rich protein (ARP), is a 20 kDa member of the ARMET family of proteins (1). The name ARMET comes from the fact that the protein was initially thought to be 50 aa longer at the N‑terminus and to contain an arginine-rich region (2-5). The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors (2-4). Human MANF is synthesized as a 179 amino acid (aa) precursor that contains a 21 aa signal sequence and a 158 aa mature chain. Mature human MANF is 99%, 98%, and 96% aa identical to mature rat, mouse and bovine MANF, respectively. MANF is localized to the endoplasmic reticulum (ER) and Golgi apparatus, and is also secreted (5). In the CNS, MANF selectively protects nigral dopaminergic neurons, versus GABAergic or serotonergic neurons, which suggests that MANF may be indicated for the treatment of Parkinson’s disease (1). MANF is also one of the 12 commonly unfolded protein response (UPR)-upregulated genes (5). One study showed that MANF plays an important role in protecting cells against tunicamycin and thapsigargin-induced cell death (5). Loss of MANF renders cells more susceptible to those drugs, but also increases cell proliferation and decreases cell size (5). Another study showed that MANF is an endoplasmic reticulum stress response (ERSR) gene in the heart that can be induced and secreted in response to ER stresses, including ischemia, and that extracellular MANF may protect cardiac myocytes in an autocrine and paracrine manner (6).
- Petrova, P.S. et al. (2003) J. Mol. Neurosci. 20:173.
- Shridhar, V. et al. (1996) Oncogene 12:1931.
- Shridhar, R. et al. (1996) Cancer Res. 56:5576.
- Shridhar, V. et al. (1997) Oncogene 14:2213.
- Apostolou, A. et al. (2008) Exp. Cell Res. 314:2454.
- Tadimalla, A. et al. (2008) Circ. Res. 103:1249.
Product Datasheets
Citations for Human MANF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Increased circulating concentrations of mesencephalic astrocyte-derived neurotrophic factor in children with type 1 diabetes
Authors: Emilia Galli, Taina Härkönen, Markus T. Sainio, Mart Ustav, Urve Toots, Arto Urtti et al.
Scientific Reports
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MANF protein expression is upregulated in immune cells in the ischemic human brain and systemic recombinant MANF delivery in rat ischemic stroke model demonstrates anti-inflammatory effects
Authors: Anttila, JE;Mattila, OS;Liew, HK;Mätlik, K;Mervaala, E;Lindholm, P;Lindahl, M;Lindsberg, PJ;Tseng, KY;Airavaara, M;
Acta neuropathologica communications
Species: Rat, Mouse
Sample Types: Serum, Tissue Homogenates
Applications: ELISA Capture -
MANF regulates neuronal survival and UPR through its ER-located receptor IRE1alpha
Authors: V Kovaleva, LY Yu, L Ivanova, O Shpironok, J Nam, A Eesmaa, EP Kumpula, S Sakson, U Toots, M Ustav, JT Huiskonen, MH Voutilaine, P Lindholm, M Karelson, M Saarma
Cell Reports, 2023-02-03;42(2):112066.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
CDNF Interacts with ER Chaperones and Requires UPR Sensors to Promote Neuronal Survival
Authors: A Eesmaa, LY Yu, H Göös, T Danilova, K Nõges, E Pakarinen, M Varjosalo, M Lindahl, P Lindholm, M Saarma
International Journal of Molecular Sciences, 2022-08-22;23(16):.
Species: Human, Mouse
Sample Types: Recombinant Protein, Tissue Homogenates
Applications: ELISA Capture -
Human-Specific Regulation of Neurotrophic Factors MANF and CDNF by microRNAs
Authors: J Konovalova, D Gerasymchu, SN Arroyo, S Kluske, F Mastroiann, AV Pereyra, A Domanskyi
International Journal of Molecular Sciences, 2021-09-07;22(18):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
Mesencephalic Astrocyte-Derived Neurotrophic Factor Is Upregulated with Therapeutic Fasting in Humans and Diet Fat Withdrawal in Obese Mice
Authors: E Galli, J Rossi, T Neumann, JO Andressoo, S Drinda, P Lindholm
Sci Rep, 2019-10-04;9(1):14318.
Species: Human, Mouse
Sample Types: Plasma, Serum
Applications: ELISA Capture -
Increased Serum Levels of Mesencephalic Astrocyte-Derived Neurotrophic Factor in Subjects With Parkinson's Disease
Authors: E Galli, A Planken, L Kadastik-E, M Saarma, P Taba, P Lindholm
Front Neurosci, 2019-09-04;13(0):929.
Species: Human
Sample Types: Serum
Applications: ELISA Capture -
Mesencephalic Astrocyte-derived Neurotrophic Factor Protects the Heart from Ischemic Damage and Is Selectively Secreted upon Sarco/endoplasmic Reticulum Calcium Depletion.
Authors: Glembotski CC, Thuerauf DJ, Huang C
J. Biol. Chem., 2012-05-25;287(31):25893-904.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Mesencephalic astrocyte-derived neurotrophic factor is an ischemia-inducible secreted endoplasmic reticulum stress response protein in the heart.
Authors: Tadimalla A, Belmont PJ, Thuerauf DJ, Glassy MS, Martindale JJ, Gude N, Sussman MA, Glembotski CC
Circ. Res., 2008-10-16;103(11):1249-58.
Species: Rat
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) Is Highly Expressed in Mouse Tissues With Metabolic Function
Authors: Tatiana Danilova, Emilia Galli, Emmi Pakarinen, Erik Palm, Päivi Lindholm, Mart Saarma et al.
Front Endocrinol (Lausanne)
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