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Human MAP3K8/Tpl2/COT Antibody

Catalog #: MAB4586
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4586
MAB4586-SP

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Detection of Human MAP3K8/Tpl2/COT by Western Blot.
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Human MAP3K8/Tpl2/COT Antibody Summary

Species Reactivity
Human
Specificity
Detects human MAP3K8/Tpl2/COT in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 634130
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MAP3K8/Tpl2/COT
Gln173-Gln395
Accession # P41279
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MAP3K8/Tpl2/COT antibody by Western Blot. View Larger

Detection of Human MAP3K8/Tpl2/COT by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MO7e human megakaryocytic leukemic cell line. PVDF Membrane was probed with 1 µg/mL of Human MAP3K8/Tpl2/COT Monoclonal Antibody (Catalog # MAB4586) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for MAP3K8/Tpl2/COT at approximately 58 and 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAP3K8/Tpl2/COT

MAP3K8, also called Tpl2 (tumor progression locus 2) or COT (Cancer Osaka Thyroid oncogene) is a cytosolic Ser/Thr kinase. MAP3K8 is one of the MAP kinase kinase (MAPKK) kinases that regulates the ERK1/ERK2 pathway in response to IL-1, facilitating IL-8 and MIP-1 beta production. MAP3K8 activates I kappa B kinases and induces the nuclear production of NF-kappa B. Human MAP3K8 shares 97% and 98% amino acid (aa) identity with mouse and rat MAP3K8, respectively, within the region used as an immunogen. Alternative start sites can give a 58 kDa (467 aa) isoform with stronger activity but shorter half-life than a 52 kDa (397 aa) isoform.

Long Name
Mitogen-activated Protein Kinase Kinase Kinase 8
Entrez Gene IDs
1326 (Human); 26410 (Mouse); 116596 (Rat)
Alternate Names
Cancer Osaka thyroid oncogene; c-COT; cot (cancer Osaka thyroid) oncogene; COT; COTMEKK8; EC 2.7.11.25; EST; ESTF; ESTFLJ10486; Ewing sarcoma transformant; MAP3K8; mitogen-activated protein kinase kinase kinase 8; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; Serine/threonine-protein kinase cot; TPL2; Tpl-2; Tumor progression locus 2; tumor progression locus-2

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Citation for Human MAP3K8/Tpl2/COT Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Identification of targetable recurrent MAP3K8 rearrangements in melanomas lacking known driver mutations
    Authors: BD Lehmann, TM Shaver, DB Johnson, Z Li, PI Gonzalez-E, V Sanchez, Y Shyr, ME Sanders, JA Pietenpol
    Mol. Cancer Res., 2019-06-11;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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