Human Matrilin-2 Antibody

Matrilin‑2 in U2OS Human Cell Line. Matrilin‑2 was detected in immersion fixed U2OS human osteosarcoma cell line using Goat Anti-Human Matrilin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3044) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Mouse Matrilin-2 by Immunohistochemistry Chemical inhibition of LOX blocks tumour growth and metastasis.(a) Tumour growth in vehicle or CCT365623-treated MMTV-PyMT mice. Data are represented as mean±s.d. from six mice per group. **P<0.01, Student's t-test. (b) H&E stained lungs from vehicle or CCT365623 treated MMTV-PyMT mice. Scale bar, 100 μm. (c) Quantification of lung metastasis in mice from a. All data are presented as mean±range, n=6 animals. **P<0.05, Mann–Whitney analysis. (d) Tumour growth in water or BAPN-treated MMTV-PyMT mice. Data are represented as mean±s.d. from seven mice per group. **P<0.01, Student's t-test. (e) H&E stained lungs from water or BAPN-treated MMTV-PyMT mice. Scale bar, 100 μm. (f) Quantification of lung metastasis in mice from d. All data are presented as mean±range, n=7 animals. **P<0.05, Mann–Whitney analysis. (g,h) Confocal photomicrographs of MATN2 (red) and EGFR (green) staining in primary and metastatic tumours from vehicle, CCT365623, water or BAPN-treated MMTV-PyMT mice. Scale bars, 20 μm. (i–l) Quantification of MATN2 and EGFR staining in primary or metastatic tumours from g and h. All data are represented as mean±s.e.m. Samples from six vehicle or CCT365623 and seven water or BAPN-treated mice were analysed. **P<0.01, Student's t-test. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14909), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Matrilin-2 by Immunohistochemistry LOX controls EGF-dependent cell proliferation in vitro and tumour growth in vivo.(a,b) Graphs showing cell proliferation in control (shCtl) and LOX-depleted (shLOX A,B) MDA-MB-231 (a) and U87 (b) cells grown in 10% FBS (serum) or serum-free medium (serum free) and treated with PBS, rhMATN2 (500 ng ml−1) and EGF (50 ng ml−1) as indicated. All data are represented as mean±s.d. from four independent experiments. **P<0.01, Student's t-test. (c) Kaplan–Meier survival plot for mice following tail vein injection of control (shCtl) or LOX-depleted (shLOX A) MDA-MB-231 cells. Seven mice were used in each group. (d) H&E staining of lungs from mice in c. Scale bar, 500 μm. (e) Quantification of lung deposits in mice. Data is represented as mean±s.d. from seven samples. **P<0.05, Mann–Whitney analysis. (f) Photomicrographs for MATN2 (red) and EGFR (green) in lung deposits. Scale bar, 10 μm. (g) Quantification of samples in f. Data is represented as mean±s.e.m. from six samples. **P<0.01, Student's t-test. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14909), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Matrilin-2 by Immunohistochemistry Chemical inhibition of LOX blocks tumour growth and metastasis.(a) Tumour growth in vehicle or CCT365623-treated MMTV-PyMT mice. Data are represented as mean±s.d. from six mice per group. **P<0.01, Student's t-test. (b) H&E stained lungs from vehicle or CCT365623 treated MMTV-PyMT mice. Scale bar, 100 μm. (c) Quantification of lung metastasis in mice from a. All data are presented as mean±range, n=6 animals. **P<0.05, Mann–Whitney analysis. (d) Tumour growth in water or BAPN-treated MMTV-PyMT mice. Data are represented as mean±s.d. from seven mice per group. **P<0.01, Student's t-test. (e) H&E stained lungs from water or BAPN-treated MMTV-PyMT mice. Scale bar, 100 μm. (f) Quantification of lung metastasis in mice from d. All data are presented as mean±range, n=7 animals. **P<0.05, Mann–Whitney analysis. (g,h) Confocal photomicrographs of MATN2 (red) and EGFR (green) staining in primary and metastatic tumours from vehicle, CCT365623, water or BAPN-treated MMTV-PyMT mice. Scale bars, 20 μm. (i–l) Quantification of MATN2 and EGFR staining in primary or metastatic tumours from g and h. All data are represented as mean±s.e.m. Samples from six vehicle or CCT365623 and seven water or BAPN-treated mice were analysed. **P<0.01, Student's t-test. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14909), licensed under a CC-BY license. Not internally tested by R&D Systems.