Human MBL Antibody

Name: Human MBL Antibody
Brand: R&D Systems
SKU: AF2307
Rating: 4.3 (3 reviews)

Catalog #: AF2307
9 Citations 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2307
AF2307-SP

All MBL Antibodies

Product Details

Citations (9)

FAQs

Supplemental Products

Reviews (3)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MBL Antibody Summary

Species Reactivity

Human

Specificity

Detects human MBL in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) MBL and rmMBL‑2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MBL
Glu21-Ile248
Accession # AAH96182

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human MBL (Catalog # 2307-MB)

Human MBL Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Human MBL Biotinylated Antibody (Catalog # BAF2307)

Standard: Recombinant Human MBL Protein (Catalog # 2307-MB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MBL

MBL belongs to the collectin family of innate immune defense proteins, which bind to an array of carbohydrate patterns on pathogen surfaces. MBL homotrimerizes to form a structural unit that further associates into oligomeric structures. Circulating MBL is complexed with three MBL-associated serine proteases (MASPs). Human MBL shares approximately 60% amino acid sequence identity with mouse MBL1 or MBL2.

References

Long Name

Mannose Binding Lectin

Entrez Gene IDs

4153 (Human)

Alternate Names

COLEC1; COLEC1collectin-1; Collectin-1; HSMBPC; Mannan-binding protein; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin (protein C) 2, soluble; Mannose-binding lectin; mannose-binding protein C; MBL; MBL2; MBL2D; MBLmannan-binding lectin; MBP; MBP1; MBP1mannose-binding lectin 2, soluble (opsonic defect); MBP-C; MGC116832; MGC116833

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human MBL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Changes in surface glycosylation and glycocalyx shedding in Trichobilharzia regenti (Schistosomatidae) during the transformation of cercaria to schistosomulum
Authors: Jana Řimnáčová, Libor Mikeš, Libuše Turjanicová, Jana Bulantová, Petr Horák
PLOS ONE
Mannose-Binding Lectin is Associated with Thrombosis and Coagulopathy in Critically Ill COVID-19 Patients
Authors: Michael Hultström, Michael Hultström, Barbro Eriksson, Barbro Persson, Kristina Nilsson Lipcsey, Kristina Nilsson Nilsson et al.
Thrombosis and Haemostasis
Mannan-Binding Lectin Suppresses Peptidoglycan-Induced TLR2 Activation and Inflammatory Responses
Authors: F Wang, Y Li, C Yang, Y Mu, Y Wang, W Zhang, Y Yang, C Chen, S Song, Z Shen, W Wang, J Li, J Zhai, K Guo, R Sun, L Yu, M Wang
Mediators Inflamm., 2019-01-09;2019(0):1349784.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Moraxella catarrhalis Evades Host Innate Immunity via Targeting Cartilage Oligomeric Matrix Protein.
Authors: Liu G, Gradstedt H, Ermert D, Englund E, Singh B, Su Y, Johansson M, Aspberg A, Agarwal V, Riesbeck K, Blom A
J Immunol, 2015-12-28;196(3):1249-58.
Species: Bacteria - Moraxella catarrhalis
Sample Types: Whole Cells
Applications: Flow Cytometry
Regulation of complement by cartilage oligomeric matrix protein allows for a novel molecular diagnostic principle in rheumatoid arthritis
Authors: Kaisa E. Happonen, Tore Saxne, Anders Aspberg, Matthias Mörgelin, Dick Heinegård, Anna M. Blom
Arthritis & Rheumatism
Mannan-binding lectin inhibits Candida albicans-induced cellular responses in PMA-activated THP-1 cells through Toll-like receptor 2 and Toll-like receptor 4.
Authors: Wang M, Wang F, Yang J, Zhao D, Wang H, Shao F, Wang W, Sun R, Ling M, Zhai J, Song S
PLoS ONE, 2013-12-31;8(12):e83517.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
A metalloproteinase karilysin present in the majority of Tannerella forsythia isolates inhibits all pathways of the complement system.
Authors: Jusko M, Potempa J, Karim AY, Ksiazek M, Riesbeck K, Garred P, Eick S, Blom AM
J. Immunol., 2012-01-27;188(5):2338-49.
Species: Human
Sample Types: Serum
Applications: ELISA Development
Scabies mite peritrophins are potential targets of human host innate immunity.
Authors: Mika A, Goh P, Holt DC
PLoS Negl Trop Dis, 2011-09-27;5(9):e1331.
Species: Human
Sample Types: Serum
Applications: Western Blot
Serglycin inhibits the classical and lectin pathways of complement via its glycosaminoglycan chains: implications for multiple myeloma.
Authors: Skliris A, Happonen KE, Terpos E, Labropoulou V, Borset M, Heinegard D, Blom AM, Theocharis AD
Eur. J. Immunol., 2010-12-30;41(2):437-49.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development