Human Melanopsin Antibody

Catalog #: MAB10497 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10497-100
MAB10497-SP

Detection of Human Melanopsin by Western Blot.

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All Melanopsin Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Melanopsin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Melanopsin in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 628737

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Melanopsin
Accession # Q9UHM6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

NS0 mouse myeloma cell line transfected with human Melanopsin

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Melanopsin by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human Melanopsin. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Melanopsin Monoclonal Antibody (Catalog # MAB10497) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Melanopsin at approximately 200-350 kDa (as indicated). GAPDH is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry

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Detection of Melanopsin in HEK293 Human Cell Line Transfected with Human Melanopsin and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) Human Melanopsin or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Melanopsin Monoclonal Antibody (Catalog # MAB10497) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Melanopsin

Melanopsin is an opsin-like protein found in the retinal ganglion cells of mammals. Melanopsin is believed to be part of a secondary optical system that parallels that of the classic rod and cone system. This second system reports directly to the suprachiasmatic nucleus and is responsible for regulation of circadian rhythms. Melanopsin is believed to be the primary photopigment responsible for the regulation of these circadian rhythms, and Melanopsin knockout mice have been generated which demonstrate decreased capacity to entrain to light and dark cycles.

Long Name

Melanopsin

Entrez Gene IDs

94233 (Human); 30044 (Mouse)

Alternate Names

Melanopsin; MGC142118; MOP; OPN4; opsin 4; Opsin-4