Human Melusin Antibody

Catalog #: AF2740 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2740
AF2740-SP

All Melusin Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Melusin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Melusin in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Melusin
Ser2-Glu347
Accession # Q9UKP3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Melusin

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human skeletal muscle

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Melusin

Melusin is a 38 kDa cytoplasmic protein that interacts specifically with the cytoplasmic domain of the beta 1 integrin subunit. It is expressed solely in striated muscle where it detects mechanical stress. The 347 amino acid residue human Melusin contains two amino terminal cysteine-rich repeats that are separated by an intervening sequence at the extreme carboxy-terminal. Melusin has a stretch of negatively charged residues (aspartic and glutamic acids). Human Melusin shares 93%, 92% and 96% amino acid sequence homology with rat, mouse and canine Melusin, respectively.

Entrez Gene IDs

26548 (Human)

Alternate Names

CHORDC3; integrin beta 1 binding protein (melusin) 2; integrin beta-1-binding protein 2; ITGB1BP; ITGB1BP2; Melusin; MGC119214