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Human MESDC2 Antibody

Catalog #: AF5577 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5577
AF5577-SP
Detection of Human MESDC2 by Western Blot.
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Human MESDC2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MESDC2 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross‑reactivity with recombinant mouse MESDC2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MESDC2
Ala34-Leu234
Accession # Q14696
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Perfusion fixed paraffin-embedded sections of mouse testis

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MESDC2 antibody by Western Blot. View Larger

Detection of Human MESDC2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MESDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5577) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MESDC2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MESDC2

Mesoderm development candidate gene 2 (MESDC2), also known as Boca in drosophila, is a 26 kDa protein (predicted) that is required for formation of the primitive streak and mesoderm during embryogenesis. MESDC2 binds to the Wnt co-receptors LRP5 and LRP6 in the endoplasmic reticulum and is required for their proper folding and cell surface expression. Within aa 34‑234, human MESDC2 shares 87% aa sequence identity with mouse and rat MESDC2, respectively.

Long Name
Mesoderm Development Candidate 2
Entrez Gene IDs
23184 (Human)
Alternate Names
BOCA; KIAA0081Renal carcinoma antigen NY-REN-61; MESDC2; MESDLDLR chaperone MESD; mesoderm development candidate 2Mesoderm development protein

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