Human MESP1 Antibody Summary
Met1-Gln85
Accession # Q9BRJ9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human MESP1 by Western Blot. Western blot shows lysates of Mouse ES cells transfected with human MESP1 untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MESP1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. Cells provided courtesy of Michael Kyba's lab, University of Minnesota.
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Detection of Human MESP1 by Western Blot. Western blot shows lysates of H1 human embryonic stem cells untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MESP1 at approximately 30 kDa (as indicated). Image provided courtesy of Michael Kyba's lab, University of Minnesota.
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Human MESP1 in H1 Human Embryonic Stem Cells. MESP1 was detected in fixed H1 human embryonic stem cells untreated (lower panel) or treated (upper panel) with Doxycycline using Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219). Cells were stained using anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Image provided courtesy of Michael Kyba's lab, University of Minnesota.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MESP1
Mesoderm posterior protein 1 (MESP1) is a 268 amino acids protein that in humans is encoded by the MESP1 gene. MESP1 was first identified in transcripts enriched in the posterior region of the mouse embryo at embryonic day E7 to E7.5. Lineage tracing in mice showed that MESP1 represents the earliest marker of cardiac progenitors and directs multipotential cardiovascular cell fates, patterning mesoderm into cardiac, hematopoietic, or skeletal myogenic progenitors in a context-dependent manner.
Product Datasheets
Citation for Human MESP1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Spatial Stem Cell Fate Engineering via Facile Morphogen Localization
Authors: Jin G, Floy ME, Simmons AD et al.
Advanced healthcare materials
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