Home / Methionine Aminopeptidase 1/METAP1 / Human Methionine Aminopeptidase 1/
METAP1 Antibody

Human Methionine Aminopeptidase 1/
METAP1 Antibody

Catalog #: AF3537 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3537
AF3537-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Methionine Aminopeptidase 1/
METAP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Methionine Aminopeptidase 1/METAP1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Methionine Aminopeptidase 1/METAP1
His52-Phe386
Accession # P53582
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Methionine Aminopeptidase 1 (Catalog # 3537-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Methionine Aminopeptidase 1 (Catalog # 3537-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Methionine Aminopeptidase 1/METAP1

The human METAP1 gene encodes Methionine Aminopeptidase 1, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). Inhibition of METAPs provides a novel strategy in developing anti-cancer drugs (3). The purified rhMETAP1 consists of amino acid residues 52/53 to 386. A previous report showed that the N-terminal 89 amino acid region was not essential for catalytic activity (4).

References
  1. Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta. 1477:157.
  2. Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
  3. Liu, S. et al. (1998) Science 282:1324.
  4. Addlagatta, A. et al. (2005) Biochemistry 44:14741.
Entrez Gene IDs
23173 (Human); 75624 (Mouse)
Alternate Names
DKFZp781C0419; EC 3.4.11.18; KIAA0094MAP 1; MAP1; MAP1A; MetAP 1; MetAP1; MetAP1A; Methionine Aminopeptidase 1; methionyl aminopeptidase 1; Peptidase M 1

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METAP1 Antibody

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