Human Methionine Aminopeptidase 1D/MAP1D Antibody

Catalog #: AF4714 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4714
AF4714-SP

All Methionine Aminopeptidase 1D/MAP1D Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Methionine Aminopeptidase 1D/MAP1D Antibody Summary

Species Reactivity

Human

Specificity

Detects human Methionine Aminopeptidase 1D/MAP1D in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Methionine Aminopeptidase 1D/MAP1D
Arg44-Ala335
Accession # Q6UB28

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Methionine Aminopeptidase 1D/MAP1D (Catalog # 4714-ZN)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Methionine Aminopeptidase 1D/MAP1D (Catalog # 4714-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Methionine Aminopeptidase 1D/MAP1D

Methionine Aminopeptidase 1D (MAP1D) is a member of the Methionine Aminopeptidase family (MetAPs) (1). MetAPs catalyze the removal of the initiator methionine residue from nascent peptides (2) and are essential for cell growth (3). MAP1D is localized in mitochondria (4) and is over-expressed in colon cancer cell lines and colon tumors (1). The N-terminal leader peptide (residue 1‑43) of MAP1D has been shown to be responsible for targeting to mitochondria (4).

References

Leszczyniecka, M. et al. (2006) Oncogene 25:3471.
Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta. 1477:157.
Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
Serero, A. et al. (2003) J. Biol. Chem. 278:52953.

Entrez Gene IDs

254042 (Human)

Alternate Names

EC 3.4.11.18; MAP1D; MAP1DCDS of metAP-3 within PCR fragment; MetAP1D; Metap1l; Methionine Aminopeptidase 1D; methionine aminopeptidase 1D, mitochondrial; methionyl aminopeptidase type 1D (mitochondrial); Methionyl aminopeptidase type 1D, mitochondrial