Human MFNG Antibody

Catalog #: AF6355 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6355
AF6355-SP

Detection of Human MFNG by Western Blot.

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Human MFNG Antibody Summary

Species Reactivity

Human

Specificity

Detects human MFNG in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MFNG
Gly37-Arg321
Accession # O00587

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human MFNG, see our available Western blot detection antibodies.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MFNG by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human MFNG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6355) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MFNG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MFNG

MFNG (Manic fringe N-acetylglucosaminyltransferase; also O-fucosylpeptide 3-beta -N-acetylglucosaminyltransferase) is a 52 - 55 kDa member of the glucosyltransferase 31 family. It is a Golgi membrane protein that transfers N-acetylglucosamine to an O-linked fucose residue on Notch. Activity on Notch increases Delta-1 induced signaling while suppressing Jagged-1 signaling. MFNG is found in fetal pancreatic endocrine progenitor cells and immature ventricular zone neurons. Human MFNG is a 321 amino acid (aa) type II transmembrane protein. It contains a short 7 aa cytoplasmic region, plus a 294 aa luminal domain (aa 28-321). There are two potential splice variants, one that shows a 15 aa substitution for aa 104-321, and another that contains a three aa substitution for aa 86-102. Over aa 37-321, human MFNG shares 85% aa identity with mouse MFNG.

Long Name

Manic Fringe O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Entrez Gene IDs

4242 (Human)

Alternate Names

beta-1,3-N-acetylglucosaminyltransferase manic fringe; EC 2.4.1.222; manic fringe (Drosophila) homolog; manic fringe homolog (Drosophila); MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; MFNG; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase