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Human MFRP C-Terminus Antibody

Catalog #: AF1915
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1915
AF1915-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MFRP C-Terminus Antibody Summary

Species Reactivity
Human
Specificity
Detects human MFRP C-Terminus in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, recombinant human Frizzled-5, rmFrizzled-6, rmFrizzled-7, rmFrizzled-8, and rmFrizzled-9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MFRP
Ser101-Pro579
Accession # Q9BY79
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human MFRP (Catalog # 1915-MF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MFRP

MFRP (membrane-type frizzled-related protein) is a 65 kDa, type II transmembrane protein related to both Tolloid proteases and frizzled-domain containing Wnt pathway proteins (1‑4). Human MFRP is 579 amino acids (aa) in length (3, 5, 6). It contains a 69 aa cytoplasmic region, a 21 aa transmembrane segment, and a 489 aa extracellular domain (ECD). The ECD is characterized by the presence of two LDLR class A repeats, two CUB domains, and a C-terminal cysteine-rich/frizzled domain. The mRNA for MFRP is highly unusual in that it is dicistronic; that is, it contains two independent ORFs, one for MFRP and one for a functionally-related protein termed CTRP5/C1qTNF5 (4, 7). CTRP5 is a secreted, 25 kDa short-chain collagen that contains a C1q-type domain (6, 8). In prokaryotes, polycistronic transcripts exist that contain functionally-interactive molecules. This would also appear to be the case for MFRP and CTRP5. CTRP5 is suggested to bind to membrane MFRP via the C1q and CUB domains, respectively. This is positioned to generate a receptor-coreceptor complex that binds select Wnts such as Wnt-1 and/or Wnt-10b (4, 6, 7). MFRP has multiple documented mutations. In human, these are associated with hyperopia (severe farsightedness). The mutations result in premature truncations (3, 9). MFRP is expressed in retinal pigment epithelium, ciliary epithelium, and keratinocytes (4, 7, 10, 11). Human MFRP ECD is 70% aa identical to mouse ECD.

References
  1. Katoh, M. and M. Katoh (2005) Oncol. Rep. 14:1381.
  2. Jones, S.E. and C. Jomary (2002) BioEssays 24:811.
  3. Sundin, O.H. et al. (2005) Proc. Natl. Acad. Sci. USA 102:9553.
  4. Mandal, M.N.A. et al. (2006) Invest. Ophthalmol. Vis. Sci. 47:5514.
  5. Katoh, M. (2001) Biochem. Biophys. Res. Commun. 282:116.
  6. Shu, X. et al. (2006) Hum. Mol. Genet. 15:1680.
  7. Kameya, S. et al. (2002) Hum. Mol. Genet. 11:1879.
  8. Hayward, C. et al. (2003) Hum. Mol. Genet. 12:2657.
  9. Ayala-Ramirez, R. et al. (2006) Mol. Vis. 12:1483.
  10. Yang, L. et al. (2006) J. Dermatol. Sci. 42:111.
  11. Ayyagari, R. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46:3363.
Long Name
Membrane Frizzled-related Protein
Entrez Gene IDs
83552 (Human); 259172 (Mouse)
Alternate Names
MCOP5; membrane frizzled-related protein; Membrane-type frizzled-related protein; MFRP; MGC32938; NNO2; rd6

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Citations for Human MFRP C-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. AMPK modulation ameliorates dominant disease phenotypes of CTRP5 variant in retinal degeneration
    Authors: Kiyoharu J. Miyagishima, Ruchi Sharma, Malika Nimmagadda, Katharina Clore-Gronenborn, Zoya Qureshy, Davide Ortolan et al.
    Communications Biology
  2. ADIPOR1 is essential for vision and its RPE expression is lost in the Mfrprd6 mouse
    Authors: VM Sluch, A Banks, H Li, MA Crowley, V Davis, C Xiang, J Yang, JT Demirs, J Vrouvliani, B Leehy, S Hanks, AM Hyman, J Aranda, B Chang, CE Bigelow, DS Rice
    Sci Rep, 2018-09-25;8(1):14339.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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