Human mGluR7 Antibody

Catalog #: MAB4405 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4405
MAB4405-SP

Detection of mGluR7 in A172 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human mGluR7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human mGluR7. Stains human mGluR7 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2A Clone # 437310

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human mGluR7
Gln35-Ile915
Accession # Q14831

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of mGluR7 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Human mGluR7 Monoclonal Antibody (Catalog # MAB4405, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR7

Metabotropic glutamate receptor 7 (mGluR7), also called GRM7 (G protein-coupled metabotropic glutamate receptor 7) is a 102 kDa member of the GRM family of 7‑transmembrane G protein-coupled receptors. mGluR7 is concentrated in the hippocampus and the amygdala and has a putative role in anxiety and spatial working memory. Within the 555 aa N-terminal extracellular domain, human mGluR7 shares > 99% aa identity with mouse, rat and dog mGluR7 and 90% aa identity with chicken mGluR7. Five isoforms (911‑924 aa) vary at the C‑terminus and are differentially expressed.

Long Name

Metabotropic Glutamate Receptor 7

Entrez Gene IDs

2917 (Human); 108073 (Mouse); 81672 (Rat)

Alternate Names

FLJ40498; glutamate receptor, metabotropic 7; GPRC1G; GRM7; metabotropic glutamate receptor 7; MGLU7; mGluR7; MGLUR7GPRC1GGLUR7