Human MICA/B APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB13001A-025
FAB13001A-100
Detection of MICA/B in K562 Human Cell Line by Flow Cytometry.
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Citations (1)
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Human MICA/B APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICA/B in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 159207
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human MICA
Accession # Q29983
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MICA/B antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MICA/B in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA/B APC-conjugated Monoclonal Antibody (Catalog # FAB13001A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MICA/B

MICA and MICB are stress-induced type I transmembrane proteins that function as ligands for NKG2D. They are distantly related to MHC class I proteins and are expressed on epithelial tumors and in response to infections.

Long Name
MHC Class I-related Protein A and B
Entrez Gene IDs
4276 (Human)
Alternate Names
MHC class I chain-related protein A; MHC class I chain-related protein B; MIC; MICA; MICA/B; MICB; PERB11; PERB11.1; PERB11.2

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Citation for Human MICA/B APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The diterpenoid adenanthin upregulates the expression of natural killer group 2D receptor ligands in hepatocellular carcinoma cells
    Authors: X Zhong, H Liao, S Hu, K Luo, H Zhu
    Molecular and cellular probes, 2021-07-12;59(0):101759.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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