Human MICL/CLEC12A APC-conjugated Antibody

Catalog #: FAB2946A Datasheet / COA / SDS

Clone 687317 was used by HLDA to establish CD designation

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FAB2946A

Detection of MICL/CLEC12A in Human Blood Granulocytes by Flow Cytometry.

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All MICL/CLEC12A Antibodies

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Human MICL/CLEC12A APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MICL/CLEC12A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC1, 2, 2A, 3B, 9A, 10A, 12B, 14A, rhCD302/CLEC13A recombinant mouse (rm) CLEC4B2, or rmMICL is observed.

Source

Monoclonal Mouse IgG_2B Clone # 687317

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MICL/CLEC12A
Thr67-Ala265
Accession # Q5QGZ9

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MICL/CLEC12A in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human MICL/CLEC12A APC-conjugated Mono-clonal Antibody (Catalog # FAB2946A, filled histo-gram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MICL/CLEC12A

Human MICL (myeloid inhibitory C-type lectin-like receptor; also CLL-1 and KLRL-1) is a 75 kDa member of the group V C-type lectin-like receptor family of molecules. Group V molecules have a lectin-type domain that binds non-sugar ligands. MICL is a 265 amino acid (aa) type II transmembrane (TM) glycoprotein that contains a 200 aa extracellular domain (ECD). Multiple isoforms of MICL are reported. One potentially utilizes an alternate start site that adds 10 aa to the cytoplasmic domain. There is one potential soluble form that lacks the TM segment, and two truncated ECD isoforms. Human MICL ECD is 53%, 65%, and 55% aa identical to the ECD in mouse, dog and cow, respectively.

Long Name

Myeloid Inhibitory C-type Lectin-like Receptor

Entrez Gene IDs

160364 (Human); 232413 (Mouse)

Alternate Names

CD371; CLEC12A; CLL1; CLL-1; C-type lectin domain family 12, member A; DCAL-2; MICL