Human MINA Antibody

Catalog #: MAB7476 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7476
MAB7476-SP

Detection of Human MINA by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MINA Antibody Summary

Species Reactivity

Human

Specificity

Detects human MINA in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MINA is observed.

Source

Monoclonal Mouse IgG₁ Clone # 753002

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MINA
Met1-Gly192
Accession # Q8IUF8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MINA by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and JAR human choriocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MINA Monoclonal Antibody (Catalog # MAB7476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MINA at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry

Detection of MINA antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

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Detection of MINA in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human MINA Monoclonal Antibody (Catalog # MAB7476, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MINA

MINA (myc-induced nuclear antigen; also Mina53) is a 52-54 kDa member of both the MINA53/NO66 and Jumonji C family of proteins. It expression is associated with proliferating cells, and it has been found in cytoplasm, nucleus and nucleoli. MINA appears to be induced by c-myc, and synthesized by spermatogonia, occasional squamous epithelium, naïve T cells and select cancer cells. When expressed, MINA is reported to regulate expression of genes such as HGF, EGF-R and IL-4. It may exert its regulatory activity through an intrinsic demethylase function. Mouse MINA is 465 amino acids (aa) in length. It possesses one cupin (or enzyme‑associated) region (aa 51-363) that contains a JmjC domain (aa 139-271). There are two potential isoform variants that contain either a 12 aa substitution for aa 145‑465, or a 15 aa substitution for aa 228-465. Over aa 2-192, mouse MINA shares 92% and 82% aa sequence identity with rat and human MINA, respectively.

Long Name

Myc-induced Nuclear Antigen

Entrez Gene IDs

84864 (Human); 67014 (Mouse); 266670 (Rat)

Alternate Names

DKFZp762O1912; FLJ14393; MDIG; MDIGDKFZp762O1912; MINA; Mina53; MINA53mdig; mineral dust induced gene protein; Mineral dust-induced gene protein; MYC induced nuclear antigen; MYC-induced nuclear antigen; myc-induced nuclear antigen, 53 kDa; NO52; Nucleolar protein 52