Human Mineralocorticoid R/NR3C2 Antibody

Catalog #: PP-H3122-00 Datasheet / COA / SDS

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PP-H3122-00

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Human Mineralocorticoid R/NR3C2 Antibody Summary

Species Reactivity

Human

Specificity

This antibody specifically recognizes human MR and cross-reacts with mouse and rat MR. Not yet tested in other species.

Source

Monoclonal Mouse IgG_2A Clone # H3122

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant human Mineralocorticoid R/NR3C2
aa 2-99

Formulation

A liquid formulation in physiologic saline with 0.1% NaN₃.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

This antibody can be used at 1 μg/mL under reducingconditions with the appropriate secondary reagents to detect human MR.

Immunohistochemistry

This antibody can be used at 20 - 40 μg/mL withthe appropriate secondary reagents to detect human MR.

Immunoprecipitation

Optimal dilutions should be determined by eachlaboratory.

Direct ELISA

This antibody can be used at 0.2 μg/mL with the appropriatesecondary reagents to detect human MR.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: Mineralocorticoid R/NR3C2

Mineralcorticoid Receptor (MR; NR3C2) is a member of the Steroid Receptor superfamily (AR,ER,GR,PR). Aldosterone binds to MR with a high affinity. MR is expressed in the kidney, gut, brain, pituitary, hypothalamus and heart. MR plays a critical role in regulating electrolyte and fluid balance in the kidney, as well as having specific roles in the central nervous system. The protein is expressed as a 107 kDa nuclear form. MR and GR can cooperatively form heterodimers.

Long Name

Mineralocorticoid Receptor

Entrez Gene IDs

4306 (Human)

Alternate Names

FLJ41052; MGC133092; Mineralocorticoid R; mineralocorticoid receptor 1; mineralocorticoid receptor delta; MineralocorticoidR; MLR; MLRmineralocorticoid receptor; MRMCRaldosterone receptor; NR3C2; NR3C2VIT; Nuclear receptor subfamily 3 group C member 2; nuclear receptor subfamily 3, group C, member 2