Human MIS/AMH Antibody Summary
Ala453-Arg560
Accession # NP_000470
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MIS/AMH
Müllerian inhibiting substance (MIS), also named anti-Müllerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to the Sertoli cells of fetal and postnatal testis, and to the granulosa cells of postnatal ovary (1). The human MIS gene encodes a 553 amino acid residue (aa) prepropeptide containing a signal a sequence (1‑24), a pro-region (25‑455), and the carboxyl-terminal bioactive protein (446-553) (2-4). MIS is synthesized and secreted as a disulfide-linked homodimeric pro-protein. Proteolytic cleavage is required to generate the N-terminal pro-region and the C-terminal bioactive protein, which remain associated in a non-covalent complex. Recombinant C-terminal MIS has been shown to be bioactive. However, the complex with the N-terminal pro-region showed enhanced activity (3, 5). The C-terminal region contains the seven canonical cysteine residues found in TGF-beta superfamily members. These cysteine residues are involved in inter- and intra-molecular disulfide bonds, which forms the cysteine knot structure. Human and mouse MIS share 73% and 90% aa sequence identity in their pro-region and C-terminal region, respectively. MIS induces Mullerian duct (female reproductive tract) regression during sexual differentiation in the male embryo (6). Posnatally, MIS has been shown to regulate gonadal functions (1). MIS inhibits Leydig cell proliferation and is a regulator of the initial and cyclic recruitment of ovarian follicles. MIS has also been found to have anti-proliferative effects on breast, ovarian and prostate tumor cells (7-9).
Like other TGF-beta superfamily members, MIS signals via a heteromeric receptor complex consisting of a type I and a type II receptor serine/threonine kinase. Depending on the cell context, different type I receptors (including Act RIA/ALK2, BMP RIA/ALK3, and BMP RIB/ALK6) that are shared by other TGF-beta superfamily members, have been implicated in MIS signaling (10-12). In contrast, the type II MIS receptor (MIS RII) is unique and does not bind other TGF-beta superfamily members. Upon ligand binding, MIS RII recruits the non-ligand binding type I receptor into the complex, resulting in phosphorylation the BMP-like signaling pathway effector proteins Smad1, Smad5 and Smad 8 (10-12).
- Teixeira, et al. (2001) Endocrine Rev. 22:657.
- Pepinsky, R.et al. (1988) J. Biol. Chem. 263:18961.
- Wilson, C.A. et al. (1993) Mol. Endocrinol. 7:247.
- Kurian, M.S. et al. (1995) Clin. Cancer Res. 1:343.
- Nachtigal, J.S. and H.A. Ingraham (1996) Proc. Natl. Acad. Sci. USA 93:7711.
- MacLaughlin, D.T. et al. (1991) Methods Enzymol. 35:358.
- Laurich, V.M. et al. (2002) Endocrinology 143:3351.
- McGee, E.A. et al. (2001) Biol. Reprod. 64:293.
- Segev, D.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:239.
- Josso, N and N. diClemente (2003) Trends Endo. Met. 14:91.
- Clarke, T.R. et al. (2001) Mol. Endocrinol. 15:946.
- Visser, J.A. (2003) Mol. Cell. Endocrinol. 211:65.
Product Datasheets
Citations for Human MIS/AMH Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Stage-dependent actions of antimüllerian hormone in regulating granulosa cell proliferation and follicular function in the primate ovary
Authors: Fuhua Xu, Maralee S. Lawson, Shawn P. Campbell, Olena Y. Tkachenko, Byung S. Park, Cecily V. Bishop et al.
F&S Science
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Anti-Mullerian hormone promotes pre-antral follicle growth, but inhibits antral follicle maturation and dominant follicle selection in primates.
Authors: Xu J, Bishop C, Lawson M, Park B, Xu F
Hum Reprod, 2016-05-09;31(7):1522-30.
Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
Sample Types: Whole Tissue
Applications: Bioassay -
Regulation of AMH and SCF expression in human granulosa cells by GnRH agonist and antagonist.
Authors: Dong M, Huang L, Wang W, Du M, He Z, Mo Y, Yang D
Pharmazie, 2011-06-01;66(6):436-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Anti-Müllerian hormone is produced heterogeneously in primate preantral follicles and is a potential biomarker for follicle growth and oocyte maturation in vitro
Authors: Jing Xu, Fuhua Xu, John H. Letaw, Byung S. Park, Robert P. Searles, Betsy M. Ferguson
Journal of Assisted Reproduction and Genetics
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Stage-specific modulation of anti-Müllerian hormone promotes primate follicular development and oocyte maturation in the matrix-free three-dimensional culture
Authors: Jing Xu, Maralee S. Lawson, Shoukhrat M. Mitalipov, Byung S. Park, Fuhua Xu
Fertility and Sterility
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Rapid actions of anti‐Müllerian hormone in regulating synaptic transmission and long‐term synaptic plasticity in the hippocampus
Authors: Kang Wang, Fuhua Xu, Shawn P. Campbell, Kyle D. Hart, Tyler Durham, James Maylie et al.
The FASEB Journal
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