Human MIS/AMH Antibody

Catalog #: MAB17372 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB17372
MAB17372-SP

Detection of Human MIS/AMH by Western Blot.

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All MIS/AMH Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MIS/AMH Antibody Summary

Species Reactivity

Human

Specificity

Detects human MIS/AMH in ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 805513

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MIS/AMH
Leu19-Gln450
Accession # P03971

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MIS/AMH by Western Blot. Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MIS/AMH Monoclonal Antibody (Catalog # MAB17372) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MIS/AMH at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIS/AMH

Müllerian inhibiting substance (MIS), also named anti-Müllerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to the Sertoli cells of fetal and postnatal testis, and to the granulosa cells of postnatal ovary (1). The human MIS gene encodes a 553 amino acid residue (aa) prepropeptide containing a signal a sequence (1-24), a pro-region (25-455), and the carboxyl-terminal bioactive protein (446-553) (2-4). MIS is synthesized and secreted as a disulfide-linked homodimeric pro-protein. Proteolytic cleavage is required to generate the N-terminal pro-region and the C-terminal bioactive protein, which remain associated in a non-covalent complex. Recombinant C-terminal MIS has been shown to be bioactive. However, the complex with the N-terminal pro-region showed enhanced activity (3, 5). The C-terminal region contains the seven canonical cysteine residues found in TGF-beta superfamily members. These cysteine residues are involved in inter- and intra-molecular disulfide bonds, which forms the cysteine knot structure. Human and mouse MIS share 73% and 90% aa sequence identity in their pro-region and C-terminal region, respectively. MIS induces Mullerian duct (female reproductive tract) regression during sexual differentiation in the male embryo (6). Posnatally, MIS has been shown to regulate gonadal functions (1). MIS inhibits Leydig cell proliferation and is a regulator of the initial and cyclic recruitment of ovarian follicles. MIS has also been found to have anti-proliferative effects on breast, ovarian and prostate tumor cells (7-9).

Like other TGF-beta superfamily members, MIS signals via a heteromeric receptor complex consisting of a type I and a type II receptor serine/threonine kinase. Depending on the cell context, different type I receptors (including Act RIA/ALK2, BMP RIA/ALK3, and BMP RIB/ALK6) that are shared by other TGF-beta superfamily members, have been implicated in MIS signaling (10-12). In contrast, the type II MIS receptor (MIS RII) is unique and does not bind other TGF-beta superfamily members. Upon ligand binding, MIS RII recruits the non-ligand binding type I receptor into the complex, resulting in phosphorylation the BMP-like signaling pathway effector proteins Smad1, Smad5, and Smad 8 (10-12).

References

Teixeira, et al. (2001) Endocrine Rev. 22:657.
Pepinsky, R.et al. (1988) J. Biol. Chem. 263:18961.
Wilson, C.A. et al. (1993) Mol. Endocrinol. 7:247.
Kurian, M.S. et al. (1995) Clin. Cancer Res. 1:343.
Nachtigal, J.S. and H.A. Ingraham (1996) Proc. Natl. Acad. Sci. USA 93:7711.
MacLaughlin, D.T. et al. (1991) Methods Enzymol. 35:358.
Laurich, V.M. et al. (2002) Endocrinology 143:3351.
McGee, E.A. et al. (2001) Biol. Reprod. 64:293.
Segev, D.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:239.
Josso, N and N. diClemente (2003) Trends Endo. Met. 14:91.
Clarke, T.R. et al. (2001) Mol. Endocrinol. 15:946.
Visser, J.A. (2003) Mol. Cell. Endocrinol. 211:65.

Long Name

Mullerian-inhibiting Substance/Anti-Mullerian Hormone

Entrez Gene IDs

268 (Human); 11705 (Mouse)

Alternate Names

AMH; MIF; MIS; Muellerian hormone; muellerian-inhibiting factor; muellerian-inhibiting substance; Mullerian hormone; Mullerian inhibiting factor; Mullerian inhibiting substance