Human MIS RII Antibody

Catalog #: AF4749 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4749
AF4749-SP

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Human MIS RII Antibody Summary

Species Reactivity

Human

Specificity

Detects human MIS RII in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant rat MIS RII is observed, and less than 1% cross-reactivity with recombinant human TGF-beta RI, RII, RIII, and RIIB is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MIS RII
Pro18-Ser144
Accession # Q16671

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human MIS RII Fc Chimera (Catalog # 4749-MR)

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human MIS RII Monoclonal Antibody (Catalog # MAB4749).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human MIS RII DuoSet ELISA Kit (Catalog # DY4749-05) for convenient development of a sandwich ELISA.

Immunohistochemistry

5-15 µg/mL

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.03-0.15 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Human MIS RII Fc Chimera (Catalog # 4749-MR) to immobilized Recombinant Human MIS/AMH (Catalog # 1737-MS) coated at 2 µg/mL (100 µL/well). At 5 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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MIS RII in Human Ovary. MIS RII was detected in immersion fixed paraffin-embedded sections of human ovary using Sheep Anti-Human MIS RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4749) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

ELISA

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Human MIS RII ELISA Standard Curve. Recombinant Human MIS RII protein was serially diluted 2-fold and captured by Mouse Anti-Human MIS RII Monoclonal Antibody (Catalog # MAB4749) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human MIS RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4749) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIS RII

Human MIS RII (Mullerian inhibiting substance type II receptor), also known as AMHRII (anti-Mullerian hormone type II receptor), is an 82 kDa serine/threonine receptor with a single transmembrane domain that belongs to the family of type II receptors of the TGF-beta superfamily (1). The MIS RII precursor is 573 amino acids in length, with a 17 amino acid (aa) signal sequence, a 127 aa extracellular region that also contains two potential N-linked glycosylation sites, a 26 aa transmembrane region, and a 403 aa cytoplasmic region that contains the serine/threonine kinase domain (1). Human MIS RII shares 82%, 78%, and 77% aa sequence identity with rabbit, mouse, and rat MIS RII, respectively. It is expressed in the mesenchyme surrounding the fetal Mullerian duct, in fetal and postnatal granulosa cells, and in Sertoli cells (1-6). MIS RII is a receptor for Mullerian inhibitor substance (MIS), also known as anti-Mullerian hormone (AMH), which is responsible for regression of the Mullerian duct, the anlagen of the uterus, Fallopian tubes, and upper vagina in male fetuses (1-6). Mutations in MIS RII result in persistent Mullerian duct syndrome (PMDS), an extremely rare form of pseudohermaphroditism (5, 6).

References

Imbeaud, S. et al. (1995) Nat. Genet. 11:382.
Baarends, W.M. et al. (1994) Development 120:189.
di Clemente, N. et al. (1994) Mol. Endocrinol. 8:1006.
Teixeira, J. et al. (1996) Endocrinology 137:160.
Salhi, I et al. (2004) Biochem. J. 379:785.
Imbeaud, S. et al. (1996) Hum. Mol. Genet. 5:1269.

Long Name

Mullerian-Inhibiting Substance Type II Receptor

Entrez Gene IDs

269 (Human); 110542 (Mouse); 29530 (Rat)

Alternate Names

AMH type II receptor; AMHR2; AMHREC 2.7.11.30; AMHRII; C14; MIS RII; MISR2; MISRII; MISRIIMIS type II receptor; MRII; Muellerian hormone type II receptor; Muellerian hormone type-2 receptor; Mullerian hormone receptor, type II; Mullerian inhibiting substance type II receptor