Human MIS RII Biotinylated Antibody

Catalog #: BAF4749 Datasheet / COA / SDS

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BAF4749

All MIS RII Antibodies

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Human MIS RII Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MIS RII in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant rat MIS RII is observed and less than 1% cross-reactivity with recombinant human TGF-beta RI, RII, RIII, and RIIB is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MIS RII
Pro18-Ser144
Accession # Q16671

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human MIS RII Fc Chimera (Catalog # 4749-MR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIS RII

Human MIS RII (Mullerian inhibiting substance type II receptor), also known as AMHRII (anti-Mullerian hormone type II receptor), is an 82 kDa serine/threonine receptor with a single transmembrane domain that belongs to the family of type II receptors of the TGF-beta superfamily (1). The MIS RII precursor is 573 amino acids in length, with a 17 amino acid (aa) signal sequence, a 127 aa extracellular region that also contains two potential N-linked glycosylation sites, a 26 aa transmembrane region, and a 403 aa cytoplasmic region that contains the serine/threonine kinase domain (1). Human MIS RII shares 82%, 78%, and 77% aa sequence identity with rabbit, mouse, and rat MIS RII, respectively. It is expressed in the mesenchyme surrounding the fetal Mullerian duct, in fetal and postnatal granulosa cells, and in Sertoli cells (1-6). MIS RII is a receptor for Mullerian inhibitor substance (MIS), also known as anti-Mullerian hormone (AMH), which is responsible for regression of the Mullerian duct, the anlagen of the uterus, Fallopian tubes, and upper vagina in male fetuses (1-6). Mutations in MIS RII result in persistent Mullerian duct syndrome (PMDS), an extremely rare form of pseudohermaphroditism (5, 6).

References

Imbeaud, S. et al. (1995) Nat. Genet. 11:382.
Baarends, W.M. et al. (1994) Development 120:189.
di Clemente, N. et al. (1994) Mol. Endocrinol. 8:1006.
Teixeira, J. et al. (1996) Endocrinology 137:160.
Salhi, I et al. (2004) Biochem. J. 379:785.
Imbeaud, S. et al. (1996) Hum. Mol. Genet. 5:1269.

Long Name

Mullerian-Inhibiting Substance Type II Receptor

Entrez Gene IDs

269 (Human); 110542 (Mouse); 29530 (Rat)

Alternate Names

AMH type II receptor; AMHR2; AMHREC 2.7.11.30; AMHRII; C14; MIS RII; MISR2; MISRII; MISRIIMIS type II receptor; MRII; Muellerian hormone type II receptor; Muellerian hormone type-2 receptor; Mullerian hormone receptor, type II; Mullerian inhibiting substance type II receptor