Human Mitofusin 1 Antibody Summary
Ala2-Lys77
Accession # Q8IWA4
Customers also Viewed
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human Mitofusin 1 by Western Blot. Western blot shows mitochondrial extracts of HeLa human cervical epithelial carcinoma cell line (20 µg/lane) and Raji human Burkitt's lymphoma cell line (2.5 µg/lane). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Mitofusin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7880) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Mitofusin 1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mitofusin 1
MFN-1 (Mitofusin 1; also Fzo/"fuzzy onions" homolog and Transmembrane GTPase MFN-1) is an 80-90 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. MFN-1 apparently mediates the fusion of individual mitochondria with each other through trans-interactions with adjacent mitochondrial MFN-1 and/or MFN-2. Pertubations with this process result in both a general overall fragmentation of mitochondria, and in cell-specific effects such as a reduction in oxidative phosphorylation or an imbalance in the generation of ROS. MFN-1 has two key domains. One is a coiled-coil region that mediates MFN-1:MFN-1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Human MFN-1 is a 741 amino acid (aa) two transmembrane protein that contains a cytoplasmic N- and C-terminus. The cytoplasmic N-terminus (aa 1-584) contains the GTPase domain (aa 82-286) while the cytoplasmic C-terminus possesses a critical coiled-coil motif (aa 679-734). MFN-1 is known to form oligomers, either with itself or MFN-2, and to undergo ubiquitination by MARCH5. Multiple splice variants are reported to occur. One is cytosolic and contains a four aa substitution for aa 367-741. Three others show a deletion of aa 444-554, aa 326-408 and an alternative start site at Met387, respectively. Over aa 1-77, human and mouse MFN-1 share 88% aa sequence identity. Full-length human MFN-1 and MFN-2 share 61% aa sequence identity.
Product Datasheets
Citations for Human Mitofusin 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
Lipids activate skeletal muscle mitochondrial fission and quality control networks to induce insulin resistance in humans
Authors: Christopher L. Axelrod, Ciaran E. Fealy, Melissa L. Erickson, Gangarao Davuluri, Hisashi Fujioka, Wagner S. Dantas et al.
Metabolism
-
Effect of Roux-en-Y gastric bypass on liver mitochondrial dynamics in a rat model of obesity
Authors: J Sacks, A Mulya, CE Fealy, H Huang, JD Mosinski, MR Pagadala, H Shimizu, E Batayyah, PR Schauer, SA Brethauer, JP Kirwan
Physiol Rep, 2018-02-01;6(4):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Exercise Training Remodels Human Skeletal Muscle Mitochondrial Fission and Fusion Machinery Towards a Pro-Elongation Phenotype.
Authors: Axelrod CL, Fealy CE, Mulya A, Kirwan JP.
Acta Physiol (Oxf).
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human Mitofusin 1 Antibody
There are currently no reviews for this product. Be the first to review Human Mitofusin 1 Antibody and earn rewards!
Have you used Human Mitofusin 1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
