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Human Mitofusin 1 Antibody

Catalog #: AF7880
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF7880
AF7880-SP
Detection of Human Mitofusin 1 by Western Blot.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mitofusin 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Mitofusin 1 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human Mitofusin 2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Mitofusin 1
Ala2-Lys77
Accession # Q8IWA4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Mitofusin 1 antibody by Western Blot. View Larger

Detection of Human Mitofusin 1 by Western Blot. Western blot shows mitochondrial extracts of HeLa human cervical epithelial carcinoma cell line (20 µg/lane) and Raji human Burkitt's lymphoma cell line (2.5 µg/lane). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Mitofusin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7880) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Mitofusin 1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mitofusin 1

MFN-1 (Mitofusin 1; also Fzo/"fuzzy onions" homolog and Transmembrane GTPase MFN-1) is an 80-90 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. MFN-1 apparently mediates the fusion of individual mitochondria with each other through trans-interactions with adjacent mitochondrial MFN-1 and/or MFN-2. Pertubations with this process result in both a general overall fragmentation of mitochondria, and in cell-specific effects such as a reduction in oxidative phosphorylation or an imbalance in the generation of ROS. MFN-1 has two key domains. One is a coiled-coil region that mediates MFN-1:MFN-1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Human MFN-1 is a 741 amino acid (aa) two transmembrane protein that contains a cytoplasmic N- and C-terminus. The cytoplasmic N-terminus (aa 1-584) contains the GTPase domain (aa 82-286) while the cytoplasmic C-terminus possesses a critical coiled-coil motif (aa 679-734). MFN-1 is known to form oligomers, either with itself or MFN-2, and to undergo ubiquitination by MARCH5. Multiple splice variants are reported to occur. One is cytosolic and contains a four aa substitution for aa 367-741. Three others show a deletion of aa 444-554, aa 326-408 and an alternative start site at Met387, respectively. Over aa 1-77, human and mouse MFN-1 share 88% aa sequence identity. Full-length human MFN-1 and MFN-2 share 61% aa sequence identity.

Entrez Gene IDs
55669 (Human); 67414 (Mouse); 192647 (Rat)
Alternate Names
DKFZp762F247; EC 3.6.5; EC 3.6.5.-; FLJ20693; Fzo homolog; hfzo1; hfzo2; MFN1; MGC41806; mitochondrial transmembrane GTPase Fzo-1; mitochondrial transmembrane GTPase FZO-2; Mitofusin 1; mitofusin-1; putative transmembrane GTPase; Transmembrane GTPase MFN1

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Citations for Human Mitofusin 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Lipids activate skeletal muscle mitochondrial fission and quality control networks to induce insulin resistance in humans
    Authors: Christopher L. Axelrod, Ciaran E. Fealy, Melissa L. Erickson, Gangarao Davuluri, Hisashi Fujioka, Wagner S. Dantas et al.
    Metabolism
  2. Effect of Roux-en-Y gastric bypass on liver mitochondrial dynamics in a rat model of obesity
    Authors: J Sacks, A Mulya, CE Fealy, H Huang, JD Mosinski, MR Pagadala, H Shimizu, E Batayyah, PR Schauer, SA Brethauer, JP Kirwan
    Physiol Rep, 2018-02-01;6(4):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Exercise Training Remodels Human Skeletal Muscle Mitochondrial Fission and Fusion Machinery Towards a Pro-Elongation Phenotype.
    Authors: Axelrod CL, Fealy CE, Mulya A, Kirwan JP.
    Acta Physiol (Oxf).

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