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Human MMP-12 Catalytic Domain Antibody

Catalog #: MAB919
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB919
MAB919-SP
Product Details
Citations (10)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-12 Catalytic Domain Antibody Summary

Species Reactivity
Human
Specificity
Detects human MMP‑12 Catalytic Domain. Does not recognize the C-terminal hemopexin-like domain of human MMP-12. Does not cross-react with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -13.
Source
Monoclonal Mouse IgG2B Clone # 82902
Purification
Protein A or G purified from ascites
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-12
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MMP‑12 Western Blot Standard (Catalog # WBC019)
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑12 (Catalog # 917‑MP), see our available Western blot detection antibodies
Immunoaffinity Purification
Sepharose beads coupled with this antibody have been used to immunoprecipitate and immunopurify Recombinant Human MMP-12 (Catalog # 917-MP) from conditioned cell culture media.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-12

MMP-12, also known as macrophage elastase, is a zinc and calcium dependent endopeptidase expressed constitutively in macrophages and can be induced in monocytes. MMP-12 can degrade a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, alpha 1-antitrypsin, and plasminogen. It can also activate MMP-2 and MMP-3. The MMP-12 precursor consists of a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain.

Long Name
Matrix Metalloproteinase 12
Entrez Gene IDs
4321 (Human); 17381 (Mouse)
Alternate Names
EC 3.4.24; hME; HMEEC 3.4.24.65; Macrophage elastase; macrophage metalloelastase; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP12; MMP-12

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Citations for Human MMP-12 Catalytic Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. MMP-14 Exhibits Greater Expression, Content and Activity Compared to MMP-15 in Human Renal Carcinoma
    Authors: M?ynarczyk, G;Tokarzewicz, A;Gudowska-Sawczuk, M;Mroczko, B;Novák, V;Novák, A;Mitura, P;Romanowicz, L;
    International journal of molecular sciences
    Species: Human
    Sample Types: Tissue Lysates
    Applications: Western Blot
  2. Comprehensive Gene Analysis of IgG4-Related Ophthalmic Disease Using RNA Sequencing
    Authors: M Asakage, Y Usui, N Nezu, H Shimizu, K Tsubota, K Umazume, N Yamakawa, T Umezu, H Suwanai, M Kuroda, H Goto
    J Clin Med, 2020-10-27;9(11):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling.
    Authors: Marina Beltrami-M, Amélie Vromman, Galina K Sukhova, Eduardo J Folco, Peter Libby
    PLoS ONE, 2016-03-31;0(0):1932-6203.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. Developing Potential Candidates of Preclinical Preeclampsia
    Authors: Sandra Founds, Xuemei Zeng, David Lykins, James M. Roberts
    International Journal of Molecular Sciences
  5. Fluid shear promotes chondrosarcoma cell invasion by activating matrix metalloproteinase 12 via IGF-2 and VEGF signaling pathways.
    Authors: Wang P, Chen S, Hung W, Paul C, Zhu F, Guan P, Huso D, Kontrogianni-Konstantopou A, Konstantopoulos K
    Oncogene, 2014-12-01;34(35):4558-69.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Neutralization
  6. Matrix metalloproteinase activity in pediatric acute lung injury.
    Authors: Kong MY, Gaggar A, Li Y
    Int J Med Sci, 2008-12-16;6(1):9-17.
    Species: Human
    Sample Types: Tissue Secretion
    Applications: Western Blot
  7. Macrophage-specific metalloelastase (MMP-12) truncates and inactivates ELR+ CXC chemokines and generates CCL2, -7, -8, and -13 antagonists: potential role of the macrophage in terminating polymorphonuclear leukocyte influx.
    Authors: Dean RA, Cox JH, Bellac CL, Doucet A, Starr AE, Overall CM
    Blood, 2008-07-25;112(8):3455-64.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  8. Langerhans cell histiocytosis reveals a new IL-17A-dependent pathway of dendritic cell fusion.
    Authors: Coury F, Annels N, Rivollier A, Olsson S, Santoro A, Speziani C, Azocar O, Flacher M, Djebali S, Tebib J, Brytting M, Egeler RM, Rabourdin-Combe C, Henter JI, Arico M, Delprat C
    Nat. Med., 2007-12-23;14(1):81-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Gene expression analysis of dendritic/Langerhans cells and Langerhans cell histiocytosis.
    Authors: Rust R, Kluiver J, Visser L, Harms G, Blokzijl T, Kamps W, Poppema S, Van Den Berg A
    J. Pathol., 2006-08-01;209(4):474-83.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. Human bronchial epithelial cells express and secrete MMP-12.
    Authors: Lavigne MC, Thakker P, Gunn J, Miyashiro JS, Wasserman AM, Wei SQ, Pelker JW
    Biochem. Biophys. Res. Commun., 2004-11-12;324(2):534-46.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation

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