Human MMP-12 Hemopexin Domain Antibody
Human MMP-12 Hemopexin Domain Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human MMP‑12 by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human MMP-12 Hemopexin Domain Monoclonal Antibody (Catalog # MAB917) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-12 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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MMP‑12 in Human Ovarian Cancer Tissue. MMP-12 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP-12 Hemopexin Domain Monoclonal Antibody (Catalog # MAB917) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-12
MMP-12, also known as macrophage elastase, is a zinc and calcium dependent endopeptidase expressed constitutively in macrophages and can be induced in monocytes. MMP-12 can degrade a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, alpha 1-antitrypsin, and plasminogen. It can also activate MMP-2 and MMP-3. The MMP-12 precursor consists of a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain.
Product Datasheets
Citations for Human MMP-12 Hemopexin Domain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Cell-type deconvolution with immune pathways identifies gene networks of host defense and immunopathology in leprosy
JCI Insight, 2016-09-22;1(15):e88843.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
GPI-anchored TIMP-1 treatment renders renal cell carcinoma sensitive to FAS-meditated killing.
Authors: Djafarzadeh R, Noessner E, Engelmann H
Oncogene, 2006;25(10):1496-508.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
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