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Human MMP-14/MT1-MMP Antibody

Catalog #: MAB9181
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB9181-SP
MAB9181-500
MAB9181-100
Detection of MMP-14/MT1-MMP in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
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Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-14/MT1-MMP Antibody Summary

Species Reactivity
Human
Specificity
Detects human MMP‑14/MT1‑MMP in direct ELISAs and Western blots. Does not detect E. coli-expressed recombinant human MMP-14 catalytic domain (aa 112-284).
Source
Monoclonal Mouse IgG2B Clone # 128527
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑14/MT1‑MMP
Tyr112-Ala541 (predicted)
Accession # P50281
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MMP‑14 (Catalog # 918-MP)
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of Human Prostate Cancer.
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑14 (Catalog # 918‑MP), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MMP-14/MT1-MMP antibody in MDA-MB-231 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MMP-14/MT1-MMP in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human MMP-14/MT1-MMP Monoclonal Antibody (Catalog # MAB9181, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Immunohistochemistry View Larger

Detection of MMP‑14/MT1‑MMP in Human Prostate Cancer. MMP‑14/MT1‑MMP was detected in immersion fixed paraffin-embedded sections of Human Prostate Cancer using Mouse Anti-Human MMP‑14/MT1‑MMP Monoclonal Antibody (Catalog # MAB9181) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-14/MT1-MMP

As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alpha V (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplasmic tail (2).

References
  1. Seike, M. (2003) Cancer Lett. 194:1.
  2. Sato, H. et al. (1994) Nature 370:61.
Long Name
Matrix Metalloproteinase 14/Membrane Type 1 MMP
Entrez Gene IDs
4323 (Human)
Alternate Names
EC 3.4.24; EC 3.4.24.80; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP14; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT1-MMPMTMMP1; MT-MMP 1; MT-MMP1

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Citations for Human MMP-14/MT1-MMP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs
    Authors: Hiroaki Kajiho
    EMBO Rep, 2016-06-02;0(0):.
  2. Improved contractile potential in detrusor microtissues from pediatric patients with end stage lower urinary tract dysfunction
    Authors: Gerwinn T, Salemi S, Schori LJ et al.
    Frontiers in Cell and Developmental Biology
  3. Syntaxin 7 contributes to breast cancer cell invasion by promoting invadopodia formation
    Authors: S Parveen, A Khamari, J Raju, MG Coppolino, S Datta
    Journal of Cell Science, 2022-06-28;135(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. A vasculature niche orchestrates stromal cell phenotype through PDGF signaling: Importance in human fibrotic disease
    Authors: TB Layton, L Williams, N Yang, M Zhang, C Lee, M Feldmann, G Trujillo, D Furniss, J Nanchahal
    Proceedings of the National Academy of Sciences of the United States of America, 2022-03-23;119(13):e2120336119.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: CyTof, IHC
  5. Cellular census of human fibrosis defines functionally distinct stromal cell types and states
    Authors: TB Layton, L Williams, F McCann, M Zhang, M Fritzsche, H Colin-York, M Cabrita, MTH Ng, M Feldmann, SN Sansom, D Furniss, W Xie, J Nanchahal
    Nat Commun, 2020-06-02;11(1):2768.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  6. Localization, Shedding, Regulation and Function of Aminopeptidase N/CD13 on Fibroblast like Synoviocytes
    PLoS ONE, 2016-09-22;11(9):e0162008.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  7. Selective blockade of matrix metalloprotease-14 with a monoclonal antibody abrogates invasion, angiogenesis, and tumor growth in ovarian cancer.
    Authors: Kaimal R, Aljumaily R, Tressel S, Pradhan R, Covic L, Kuliopulos A, Zarwan C, Kim Y, Sharifi S, Agarwal A
    Cancer Res, 2013-02-19;73(8):2457-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Contractile forces contribute to increased glycosylphosphatidylinositol-anchored receptor CD24-facilitated cancer cell invasion.
    Authors: Mierke CT, Bretz N, Altevogt P
    J. Biol. Chem., 2011-08-02;286(40):34858-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Integrin alpha5beta1 facilitates cancer cell invasion through enhanced contractile forces.
    Authors: Mierke CT, Frey B, Fellner M, Herrmann M, Fabry B
    J. Cell. Sci., 2011-01-11;124(0):369-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. GPI-anchored TIMP-1 treatment renders renal cell carcinoma sensitive to FAS-meditated killing.
    Authors: Djafarzadeh R, Noessner E, Engelmann H
    Oncogene, 2006;25(10):1496-508.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

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