Human MMP-25/MT6-MMP Antibody

Catalog #: MAB11422 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11422
MAB11422-SP

Detection of MMP‑25/MT6‑MMP in Jurkat Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-25/MT6-MMP Antibody Summary

Species Reactivity

Human

Specificity

Detects human MMP‑25/MT6-MMP in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 141811

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP‑25/MT6-MMP
Pro314-Cys508
Accession # Q9NPA2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of MMP-25/MT6-MMP antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

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Detection of MMP‑25/MT6‑MMP in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human MMP-25/MT6-MMP Monoclonal Antibody (Catalog # MAB11422, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-25/MT6-MMP

MMP-25, also known as MT6-MMP or Leukolysin, is a GPI-linked protease expressed by neutrophils. MMP-25 cleaves collagen type 4, gelatin, fibronectin, and fibrin.

Long Name

Matrix Metalloproteinase 25/Membrane Type 6 MMP

Entrez Gene IDs

64386 (Human)

Alternate Names

Leukolysin; matrix metallopeptidase 25; matrix metallopeptidase-like 1; matrix metalloproteinase 20; matrix metalloproteinase 25; matrix metalloproteinase-25; matrix metalloproteinase-like 1; membrane-type 6 matrix metalloproteinase; membrane-type matrix metalloproteinase 6; membrane-type-6 matrix metalloproteinase; MMP20; MMP20A; MMP25; MMP-25; MMPL1; MT6MMP; MT6-MMP; MT-MMP 6; MTMMP6; MT-MMP6