Human MMR/CD206 Antibody Summary
Leu19-Lys1383 (Thr399Ala) & (Leu407Phe)
Accession # P22897
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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MMR/CD206 in Human PBMCs. MMR/CD206 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Rat Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB2534) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMR/CD206
The human Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 190 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The human MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1371 aa extracellular region, a 21 aa transmembrane segment and a 46 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3, 4). Human to mouse, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (5). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (6). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor is recycled to the cell surface (3, 7). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).
- East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364.
- Chieppa, M. et al. (2003) J. Immunol. 171:4552.
- Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
- Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
- Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
- Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
- Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.
Product Datasheets
Citations for Human MMR/CD206 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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The ASIC3-M-CSF-M2 macrophage-positive feedback loop modulates fibroblast-to-myofibroblast differentiation in skin fibrosis pathogenesis
Authors: JJ Wu, ZL Sun, SY Liu, ZH Chen, ZD Yuan, ML Zou, YY Teng, YY Li, DY Guo, FL Yuan
Cell Death & Disease, 2022-06-06;13(6):527.
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The expression of IL17RA on sputum macrophages in asthma patients
Authors: P Nejman-Gry, M Papli?ska-, M Proboszcz, M Grabczak, J Hermanowic, R Krenke
Cytokine, 2021-04-08;0(0):155518.
Species: Human
Sample Types: Sputum
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Macrophage mannose receptor, CD206, predict prognosis in patients with pulmonary tuberculosis
Authors: Y Suzuki, M Shirai, K Asada, H Yasui, M Karayama, H Hozumi, K Furuhashi, N Enomoto, T Fujisawa, Y Nakamura, N Inui, T Shirai, H Hayakawa, T Suda
Sci Rep, 2018-09-03;8(1):13129.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer
Authors: Maija Hollmén, Sinem Karaman, Simon Schwager, Angela Lisibach, Ailsa J. Christiansen, Mikael Maksimow et al.
OncoImmunology
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ROCK-isoform-specific polarization of macrophages associated with age-related macular degeneration.
Authors: Zandi S, Nakao S, Chun K, Fiorina P, Sun D, Arita R, Zhao M, Kim E, Schueller O, Campbell S, Taher M, Melhorn M, Schering A, Gatti F, Tezza S, Xie F, Vergani A, Yoshida S, Ishikawa K, Yamaguchi M, Sasaki F, Schmidt-Ullrich R, Hata Y, Enaida H, Yuzawa M, Yokomizo T, Kim Y, Sweetnam P, Ishibashi T, Hafezi-Moghadam A
Cell Rep, 2015-02-19;10(7):1173-86.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
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