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Human MMR/CD206 APC-conjugated Antibody

Catalog #: FAB25342A
3 Citations Datasheet / COA / SDS
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FAB25342A
Detection of MMR/CD206 in Human Immature Dendritic Cells by Flow Cytometry.
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Citations (3)
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Human MMR/CD206 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse MMR is observed.
Source
Monoclonal Mouse IgG2A Clone # 685641
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206
Leu19-Lys1383 (Thr399Ala) & (Leu407Phe)
Accession # P22897
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MMR/CD206 antibody in Human Immature Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of MMR/CD206 in Human Immature Dendritic Cells by Flow Cytometry. Human monocyte-derived immature dendritic cells were stained with Mouse Anti-Human MMR/CD206 APC-conjugated Monoclonal Antibody (Catalog # FAB25342A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MMR/CD206

The human Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 190 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The human MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1371 aa extracellular region, a 21 aa transmembrane segment and a 46 aa cytoplasmic domain (4). Its extracellular region is composed of an N‑terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3, 4). Human to mouse, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N‑acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (5). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (6). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin‑coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor is recycled to the cell surface (3, 7). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

References
  1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
  2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
  3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
  4. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
  5. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  6. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
  7. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.
Long Name
Macrophage Mannose Receptor
Entrez Gene IDs
4360 (Human); 17533 (Mouse); 291327 (Rat)
Alternate Names
CD206; CLEC13D; CLEC13Dmacrophage mannose receptor 1; C-type lectin domain family 13 member D; mannose receptor, C type 1; MMR; MMRCD206 antigen; MRC1

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Citations for Human MMR/CD206 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Complement inhibitor factor H expressed by breast cancer cells differentiates CD14+ human monocytes into immunosuppressive macrophages
    Authors: Karolina I. Smolag, Christine M. Mueni, Karin Leandersson, Karin Jirström, Catharina Hagerling, Matthias Mörgelin et al.
    OncoImmunology
  2. Identification of resolvin D2 receptor mediating resolution of infections and organ protection.
    Authors: Chiang N, Dalli J, Colas R, Serhan C
    J Exp Med, 2015-07-20;212(8):1203-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. ChemR23, the receptor for chemerin and resolvin E1, is expressed and functional on M1 but not on M2 macrophages.
    Authors: Herova M, Schmid M, Gemperle C, Hersberger M
    J Immunol, 2015-01-30;194(5):2330-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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