Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody
Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody Summary
Ser2-Ser501
Accession # P00352
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 by Western Blot. Western blot shows lysates of human liver tissue, human testis tissue, and mouse liver. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Aldehyde Dehydrogenase 1-A1/ALDH1A1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Aldehyde Dehydrogenase 1-A1/ALDH1A1 in HeLa Human Cell Line. Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Aldehyde Dehydrogenase 1‑A1/ALDH1A1 in Human Prostate Cancer Tissue. Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human and Mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Aldehyde Dehydrogenase 1-A1/ALDH1A1 at approximately 57 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1
Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences (1). ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid (2). ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea (3). ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development (4). The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells (5). ALDH1A1 is a major enzyme in the oxidation of acetaldehyde, a toxic metabolite of ethanol (6).
Product Datasheets
Citations for Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Development of an arteriolar niche and self-renewal of breast cancer stem cells by lysophosphatidic acid/protein kinase D signaling
Authors: Yinan Jiang, Yichen Guo, Jinjin Hao, Rachael Guenter, Justin Lathia, Adam W. Beck et al.
Communications Biology
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Inhibitory co-transmission from midbrain dopamine neurons relies on presynaptic GABA uptake
Authors: Riccardo Melani, Nicolas X. Tritsch
Cell Reports
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Multisensory gamma stimulation mitigates the effects of demyelination induced by cuprizone in male mice
Authors: Rodrigues-Amorim, D;Bozzelli, PL;Kim, T;Liu, L;Gibson, O;Yang, CY;Murdock, MH;Galiana-Melendez, F;Schatz, B;Davison, A;Islam, MR;Shin Park, D;Raju, RM;Abdurrob, F;Nelson, AJ;Min Ren, J;Yang, V;Stokes, MP;Tsai, LH;
Nature communications
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Niche stiffness sustains cancer stemness via TAZ and NANOG phase separation
Authors: X Liu, Y Ye, L Zhu, X Xiao, B Zhou, Y Gu, H Si, H Liang, M Liu, J Li, Q Jiang, J Li, S Yu, R Ma, S Su, JY Liao, Q Zhao
Nature Communications, 2023-01-16;14(1):238.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype
Authors: W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang
Oncogene, 2022-11-22;0(0):.
Species: Xenograft
Sample Types: Whole Cells
Applications: Flow Cytometry -
Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells
Authors: J Kim, J Jeon, B Song, N Lee, S Ko, Y Cha, P Leblanc, H Seo, KS Kim
Nature Protocols, 2022-02-09;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Sox6 expression distinguishes dorsally and ventrally biased dopamine neurons in the substantia nigra with distinctive properties and embryonic origins
Authors: M Pereira Lu, M Azcorra, G Caronia-Br, JF Poulin, Z Gaertner, S Gatica, OA Moreno-Ram, N Nouri, M Dubois, YC Ma, C Ramakrishn, L Fenno, YS Kim, K Deisseroth, F Cicchetti, DA Dombeck, R Awatramani
Cell Reports, 2021-11-09;37(6):109975.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
Authors: J Wu, J Kung, J Dong, L Chang, C Xie, A Habib, S Hawes, N Yang, V Chen, Z Liu, R Evans, B Liang, L Sun, J Ding, J Yu, S Saez-Atien, B Tang, Z Khaliq, DT Lin, W Le, H Cai
Cell Rep, 2019-07-30;28(5):1167-1181.e7.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Aldehyde dehydrogenase 1-positive nigrostriatal dopaminergic fibers exhibit distinct projection pattern and dopamine release dynamics at mouse dorsal striatum
Authors: C Sgobio, J Wu, W Zheng, X Chen, J Pan, AG Salinas, MI Davis, DM Lovinger, H Cai
Sci Rep, 2017-07-13;7(1):5283.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
PMP22 Regulates Self-Renewal and Chemoresistance of Gastric Cancer Cells
Authors: W Cai, G Chen, Q Luo, J Liu, X Guo, T Zhang, F Ma, L Yuan, B Li, J Cai
Mol. Cancer Ther, 2017-03-23;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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