Human/Mouse Alkaline Phosphatase/ALPL Antibody

Catalog # Availability Size / Price Qty
AF2909
AF2909-SP
Detection of Human and Mouse Alkaline Phosphatase/ALPL by Western Blot.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse Alkaline Phosphatase/ALPL Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Alkaline Phosphatase/ALPL in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Alkaline Phosphatase/ALPL
Leu18-Phe524
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Alkaline Phosphatase/ALPL antibody by Western Blot. View Larger

Detection of Human and Mouse Alkaline Phosphatase/ALPL by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BG01V human embryonic stem cells, NTera-2 human testicular embryonic carcinoma cell line, and mouse kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Alkaline Phosphatase/ALPL

Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503‑524) is not present in the mature form.

References
  1. Le Du, M-H. and J.L. Millan (2002) J. Biol. Chem. 277:49808.
  2. Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1.
  3. Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.
Long Name
Alkaline Phosphatase Liver
Entrez Gene IDs
249 (Human); 11647 (Mouse)
Alternate Names
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935

Product Datasheets

You must select a language.

x

Citation for Human/Mouse Alkaline Phosphatase/ALPL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Brain Endothelial Cells Are Exquisite Sensors of Age-Related Circulatory Cues
    Authors: MB Chen, AC Yang, H Yousef, D Lee, W Chen, N Schaum, B Lehallier, SR Quake, T Wyss-Coray
    Cell Rep, 2020-03-31;30(13):4418-4432.e4.
    Species: Mouse
    Sample Types: Whole Tissue

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse Alkaline Phosphatase/ALPL Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse Alkaline Phosphatase/ALPL Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse Alkaline Phosphatase/ALPL Antibody
By Anonymous on 08/31/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: bone marrow Species: Mouse