Human/Mouse Amphiphysin/AMPH Antibody

Catalog #: MAB65271 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB65271
MAB65271-SP

Detection of Human and Mouse Amphiphysin/ AMPH by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Amphiphysin/AMPH Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human Amphiphysin/AMPH in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 694121

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Amphiphysin/AMPH
Asn204-Gly300
Accession # P49418

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Amphiphysin/ AMPH by Western Blot. Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Amphiphysin/AMPH Monoclonal Antibody (Catalog # MAB65271) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Amphiphysin/AMPH at approximately 128 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Amphiphysin/AMPH

AMPH (Amphiphysin; also AMPH1) is a member of the Amphiphysin family, BAR superfamily of proteins. Although its calculated MW is only 78 kDa, it runs anomalously in SDS-PAGE at 125-128 kDa. It is expressed in neurons, Sertoli cells, possibly in anterior pituitary endocrine cells and apparently cardiac muscle. It may form heterodimers with AMPH2, and in this configuration, promotes clathrin-associated endocytic vesicle formation. Furthermore, and perhaps related, AMPH also interacts with N-WASP, initiating actin polymerization. Human AMPH is 695 amino acids (aa) in length. It contains an N-terminal BAR domain (aa 24-240) that binds lipids, a central region that binds AP2 (aa 322-340) and clathrin (aa 347-386), and a C-terminal SH3 domain (aa 622-695) that binds proline-rich ligands. There is one known 108 kDa splice variant that shows a deletion of aa 425-466. Over aa 204-300, human AMPH shares 98% aa identity with mouse AMPH.

Entrez Gene IDs

273 (Human); 218038 (Mouse); 60668 (Rat)

Alternate Names

AMPH; AMPH1; amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen); amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen); amphiphysin I; Amphiphysin